Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

P Comizzoli, O Amelkina, PC Lee - Molecular Reproduction and …, 2022 - Wiley Online Library
Long‐term preservation of sperm, oocytes, and gonadal tissues at ambient temperatures
has the potential to lower the costs and simplify biobanking in human reproductive medicine …

Chimaeras, complementation, and controlling the male germline

B Oback, DA Cossey - Trends in Biotechnology, 2023 - cell.com
Animal breeding drives genetic progress mainly through the male germline. This process is
slow to respond to rapidly mounting environmental pressures that threaten sustainable food …

Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration

T Yu, Q Ao, T Ao, MA Ahmad, A Wang… - Bioengineering & …, 2023 - Wiley Online Library
Peripheral nerve regeneration after injury is still a clinical problem. The application of
autologous nerve grafting, the gold standard treatment, is greatly restricted. Acellular nerve …

Production of mouse offspring from zygotes fertilized with freeze-dried spermatids

S Wakayama, D Ito, M Ooga, T Wakayama - Scientific Reports, 2022 - nature.com
Mouse cloning by nuclear transfer using freeze-drying (FD) somatic cells is now possible,
but the success rate is significantly lower than that of FD spermatozoa. Because …

Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying

PC Lee, S Stewart, O Amelkina, H Sylvester… - Journal of Assisted …, 2023 - Springer
Purpose Trehalose is a non-permeable protectant that is the key to preserve live cells in a
dry state for potential storage at ambient temperatures. After intracellular trehalose delivery …

Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles

PC Lee, P Comizzoli - Biology of Reproduction, 2024 - academic.oup.com
Germinal vesicles are alternative targets for female fertility preservation due to their
availability and high resilience against non-physiological conditions. Preserved germinal …

Mouse somatic cell nuclear transfer: what has changed and remained unchanged in 25 years

K Inoue - Journal of Reproduction and Development, 2023 - jstage.jst.go.jp
Somatic cell nuclear transfer (SCNT) is the only reproductive technology used to produce
individuals from somatic cells by transferring them to enucleated oocytes. Although more …

L-Proline as a Cryoprotective Agent for the Preservation of Galea Spixii Skin Fibroblasts

LVC Aquino, LLV Rodrigues, SL Olindo… - Biopreservation and …, 2024 - liebertpub.com
Somatic cell biobanking is a promising strategy for developing reproductive techniques.
Although cryopreservation, a technique used for creating biobanks, has been performed on …

Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens

Y Fu, W Dang, X He, F Xu, H Huang - Bioengineering, 2022 - mdpi.com
Low-temperature preservation could effectively extend in vitro storage of biological materials
due to delayed or suspended cellular metabolism and decaying as illustrated by the …

Freeze‐Dried Mesenchymal Stem Cells: From Bench to Bedside. Review

T Paresishvili, Z Kakabadze - Advanced Biology, 2024 - Wiley Online Library
This review describes the freeze‐dried mesenchymal stem cells (MSCs) and their ability to
restore damaged tissues and organs. An analysis of the literature shows that after the …