Phototoxicity in live fluorescence microscopy, and how to avoid it

J Icha, M Weber, JC Waters, C Norden - BioEssays, 2017 - Wiley Online Library
Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …

Highlights on selected microscopy techniques to study zebrafish developmental biology

A Abu-Siniyeh, W Al-Zyoud - Laboratory Animal Research, 2020 - Springer
Bio-imaging is a tedious task when it concerns exploring cell functions, developmental
mechanisms, and other vital processes in vivo. Single-cell resolution is challenging due to …

Harnessing quantum light for microscopic biomechanical imaging of cells and tissues

T Li, V Cheburkanov, VV Yakovlev, GS Agarwal… - Proceedings of the …, 2024 - pnas.org
The biomechanical properties of cells and tissues play an important role in our fundamental
understanding of the structures and functions of biological systems at both the cellular and …

Differentiating melanoma and healthy tissues based on elasticity-specific Brillouin microspectroscopy

M Troyanova-Wood, Z Meng… - Biomedical optics …, 2019 - opg.optica.org
The main objective of the present study is to evaluate the use of Brillouin microspectroscopy
for differentiation of melanoma and normal tissues based on elasticity measurements …

Subcellular measurements of mechanical and chemical properties using dual Raman‐Brillouin microspectroscopy

Z Meng, SC Bustamante Lopez… - Journal of …, 2016 - Wiley Online Library
Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In
particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical …

Coherent anti-Stokes Raman scattering imaging of microcalcifications associated with breast cancer

GI Petrov, R Arora, VV Yakovlev - Analyst, 2021 - pubs.rsc.org
Chemical imaging of calcifications was demonstrated in the depth of a tissue. Using long
wavelength excitation, broadband coherent anti-Stokes Raman scattering and hierarchical …

Machine learning estimation of tissue optical properties

BH Hokr, JN Bixler - Scientific Reports, 2021 - nature.com
Dynamic, in vivo measurement of the optical properties of biological tissues is still an elusive
and critically important problem. Here we develop a technique for inverting a Monte Carlo …

Nonlinear Brillouin spectroscopy: what makes it a better tool for biological viscoelastic measurements

CW Ballmann, Z Meng, VV Yakovlev - Biomedical optics express, 2019 - opg.optica.org
Brillouin spectroscopy is an emerging tool in biomedical imaging and sensing. It is capable
of assessing the high-frequency viscoelastic longitudinal modulus with microscopic spatial …

Modeling focusing Gaussian beams in a turbid medium with Monte Carlo simulations

BH Hokr, JN Bixler, G Elpers, B Zollars, RJ Thomas… - Optics express, 2015 - opg.optica.org
Monte Carlo techniques are the gold standard for studying light propagation in turbid media.
Traditional Monte Carlo techniques are unable to include wave effects, such as diffraction; …

Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet‐induced obese rats

M Troyanova‐Wood, C Gobbell, Z Meng… - Journal of …, 2017 - Wiley Online Library
Obesity is becoming a leading cause of health problems world‐wide. Obesity and
overweight are associated with the structural and chemical changes in tissues; however, few …