The technological landscape and applications of single-cell multi-omics

A Baysoy, Z Bai, R Satija, R Fan - Nature Reviews Molecular Cell …, 2023 - nature.com
Single-cell multi-omics technologies and methods characterize cell states and activities by
simultaneously integrating various single-modality omics methods that profile the …

Optimized single-nucleus transcriptional profiling by combinatorial indexing

BK Martin, C Qiu, E Nichols, M Phung… - Nature protocols, 2023 - nature.com
Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for
recovering gene expression data from an exponentially scalable number of individual cells …

Into the multiverse: advances in single-cell multiomic profiling

S Ogbeide, F Giannese, L Mincarelli, IC Macaulay - Trends in Genetics, 2022 - cell.com
Single-cell transcriptomic approaches have revolutionised the study of complex biological
systems, with the routine measurement of gene expression in thousands of cells enabling …

Immunometabolism in the single-cell era

MN Artyomov, J Van den Bossche - Cell metabolism, 2020 - cell.com
Emerging research has identified metabolic pathways that are crucial for the proper
regulation of immune cells and how, when deranged, they can cause immune dysfunction …

The evolution of single-cell RNA sequencing technology and application: progress and perspectives

S Wang, ST Sun, XY Zhang, HR Ding, Y Yuan… - International Journal of …, 2023 - mdpi.com
As an emerging sequencing technology, single-cell RNA sequencing (scRNA-Seq) has
become a powerful tool for describing cell subpopulation classification and cell …

High-throughput single-cell quantification of hundreds of proteins using conventional flow cytometry and machine learning

E Becht, D Tolstrup, CA Dutertre, PA Morawski… - Science …, 2021 - science.org
Modern immunologic research increasingly requires high-dimensional analyses to
understand the complex milieu of cell types that comprise the tissue microenvironments of …

Spatial transcriptomics technology in cancer research

Q Yu, M Jiang, L Wu - Frontiers in Oncology, 2022 - frontiersin.org
In recent years, spatial transcriptomics (ST) technologies have developed rapidly and have
been widely used in constructing spatial tissue atlases and characterizing spatiotemporal …

Pooled Genome-Scale CRISPR Screens in Single Cells

D Schraivogel, LM Steinmetz… - Annual Review of …, 2023 - annualreviews.org
Assigning functions to genes and learning how to control their expression are part of the
foundation of cell biology and therapeutic development. An efficient and unbiased method to …

Spatial transcriptomics: recent developments and insights in respiratory research

WJ Wang, LX Chu, LY He, MJ Zhang, KT Dang… - Military Medical …, 2023 - Springer
The respiratory system's complex cellular heterogeneity presents unique challenges to
researchers in this field. Although bulk RNA sequencing and single-cell RNA sequencing …

Microfluidics-based single cell analysis: from transcriptomics to spatiotemporal multi-omics

J Cao, X Chen, S Huang, W Shi, Q Fan, Y Gong… - TrAC Trends in …, 2023 - Elsevier
Uncover cell composition, cellular spatial distribution, and cell-to-cell interactions in tissues
are significant for the understanding of physiological and pathological processes. Over the …