The isolation of chloramphenicol from a soil bacterium (Streptomyces venezuelae) was
reported in 1947.'Its structure was soon revealed to be simple but novel in two respects and …

The plasmid pBR322 was one of the first EK2 multipurpose cloning vectors to be designed
and constructed (ten years ago) for the efficient cloning and selection of recombinant DNA …

We have developed a new vector strategy for the insertion of foreign genes into the
genomes of gram negative bacteria not closely related to Escherichia coli. The system …

A “plant gene vector cassette” to be used in combination with various Escherichia coli gene-
cloning vectors was constructed. This cassette contains a replication and mobilization unit …

A gene bank of Sau 3A partially digested Candida albicans DNA in vector YEp13 was used
to complement a ura3 mutation (orotidine-5′-phosphate decarboxylase, OMPdecase) in S …

High-rate electron transfer toward an anode in microbial fuel cells (MFCs) has thus far not
been described for bacteria-producing soluble redox mediators. To study the mechanism of …

Members of the conserved Argonaute protein family use small RNA guides to locate their
mRNA targets and regulate gene expression and suppress mobile genetic elements in …

We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations.
The system is based on an auxotrophic mutant host of P. pastoris which is defective in …

Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We
have constructed an expression vector derived from the promoter sequence of nopaline …

The T-DNA region of Agrobacterium tumefaciens tumour-inducing plasmids of the nopaline
type1 contains a gene coding for the enzyme nopaline synthase. This gene is expressed …