Digital models in biotechnology: towards multi-scale integration and implementation
Industrial biotechnology encompasses a large area of multi-scale and multi-disciplinary
research activities. With the recent megatrend of digitalization sweeping across all …
research activities. With the recent megatrend of digitalization sweeping across all …
[HTML][HTML] Probing interdomain linkers and protein supertertiary structure in vitro and in live cells with fluorescent protein resonance energy transfer
Many proteins are composed of independently-folded domains connected by flexible linkers.
The primary sequence and length of such linkers can set the effective concentration for the …
The primary sequence and length of such linkers can set the effective concentration for the …
Correlating viscosity and molecular crowding with fluorescent nanobeads and molecular probes: in vitro and in vivo
S Lecinski, JW Shepherd, K Bunting… - Interface …, 2022 - royalsocietypublishing.org
In eukaryotes, intracellular physico-chemical properties like macromolecular crowding and
cytoplasmic viscoelasticity influence key processes such as metabolic activities, molecular …
cytoplasmic viscoelasticity influence key processes such as metabolic activities, molecular …
Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
Förster resonance energy transfer (FRET) between fluorescent proteins has become a
common platform for designing genetically encoded biosensors. For live cell imaging, the …
common platform for designing genetically encoded biosensors. For live cell imaging, the …
FRET Analysis of ionic strength sensors in the hofmeister series of salt solutions using fluorescence lifetime measurements
Living cells are complex, crowded, and dynamic and continually respond to environmental
and intracellular stimuli. They also have heterogeneous ionic strength with …
and intracellular stimuli. They also have heterogeneous ionic strength with …
Förster resonance energy transfer from Hoechst 33258 to SYBR Green I in cholesteric liquid-crystalline DNA
VN Morozov, MA Klimovich, AA Kostyukov… - Journal of …, 2022 - Elsevier
In the present work Förster resonance energy transfer (FRET) between DNA-specific
fluorescent dyes Hoechst 33258 (Ht58; donor) and SYBR Green I (SG; acceptor) is …
fluorescent dyes Hoechst 33258 (Ht58; donor) and SYBR Green I (SG; acceptor) is …
Two-photon excited-state dynamics of mEGFP-linker-mScarlet-I crowding biosensor in controlled environments
SA Mersch, S Bergman, ED Sheets… - Physical Chemistry …, 2024 - pubs.rsc.org
Macromolecular crowding affects many cellular processes such as diffusion, biochemical
reaction kinetics, protein–protein interactions, and protein folding. Mapping the …
reaction kinetics, protein–protein interactions, and protein folding. Mapping the …
Protein dynamics as a sensor for macromolecular crowding: Insights into mixed crowding
M Gupta, PK Chowdhury - Journal of Molecular Liquids, 2022 - Elsevier
Understanding the effect of mixed crowding is of prime importance with regards to
addressing the manner in which the crowded cellular interior influences the structure …
addressing the manner in which the crowded cellular interior influences the structure …
Translational diffusion, molecular brightness, and energy transfer analysis of mEGFP-linker-mScarlet–I crowding biosensor using fluorescence correlation …
SA Mersch, C McCue, A Aristidou, ED Sheets… - Physical Chemistry …, 2024 - pubs.rsc.org
Recently, we have investigated the sensitivity of an mEGFP-linker-mScarlet-I construct (GE2.
3) in response to macromolecular crowding using ensemble time-resolved two-photon (2P) …
3) in response to macromolecular crowding using ensemble time-resolved two-photon (2P) …
Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy
Eukaryotic cells exploit dynamic and compartmentalized ionic strength to impact a myriad of
biological functions such as enzyme activities, protein-protein interactions, and catalytic …
biological functions such as enzyme activities, protein-protein interactions, and catalytic …