Label-free live cell recognition and tracking for biological discoveries and translational applications

B Chen, Z Yin, BWL Ng, DM Wang, RS Tuan, R Bise… - npj Imaging, 2024 - nature.com
Label-free, live cell recognition (ie instance segmentation) and tracking using computer
vision-aided recognition can be a powerful tool that rapidly generates multi-modal readouts …

In silico labeling in cell biology: Potential and limitations

N Elmalam, LB Nedava, A Zaritsky - Current Opinion in Cell Biology, 2024 - Elsevier
In silico labeling is the computational cross-modality image translation where the output
modality is a subcellular marker that is not specifically encoded in the input image, for …

High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies

AJ Asmar, ZA Benson, AP Peskin, J Chalfoun, M Simon… - Plos one, 2024 - journals.plos.org
To facilitate the characterization of unlabeled induced pluripotent stem cells (iPSCs) during
culture and expansion, we developed an AI pipeline for nuclear segmentation and mitosis …

The use of artificial intelligence in induced pluripotent stem cell-based technology over 10-year period: A systematic scoping review

QD Vo, Y Saito, T Ida, K Nakamura, S Yuasa - Plos one, 2024 - journals.plos.org
Background Stem cell research, particularly in the domain of induced pluripotent stem cell
(iPSC) technology, has shown significant progress. The integration of artificial intelligence …

Seeing beyond Labels: Optical Technologies Reshaping Cell Culture Monitoring in Biomedicine

M Charconnet, J Plou - ACS Applied Engineering Materials, 2024 - ACS Publications
Label-free optical technologies have transformed cell culture monitoring, offering
noninvasive alternatives that preserve cellular integrity─ a critical factor in biomedical …

Perspectives on label-free microscopy of heterogeneous and dynamic biological systems

DL Pham, AA Gillette, J Riendeau… - Journal of …, 2025 - spiedigitallibrary.org
Significance Advancements in label-free microscopy could provide real-time, non-invasive
imaging with unique sources of contrast and automated standardized analysis to …

Utilizing convolutional neural networks for discriminating cancer and stromal cells in three-dimensional cell culture images with nuclei counterstain

HT Nguyen, N Pietraszek, SE Shelton… - Journal of …, 2024 - spiedigitallibrary.org
Significance Accurate cell segmentation and classification in three-dimensional (3D) images
are vital for studying live cell behavior and drug responses in 3D tissue culture. Evaluating …

Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation

DE Desa, MJ Amitrano, WL Murphy… - Biophotonics …, 2024 - spiedigitallibrary.org
Significance Heart disease is the leading cause of death in the United States, yet research is
limited by the inability to culture primary cardiac cells. Cardiomyocytes (CMs) derived from …

Cell-context dependent in silico organelle localization in label-free microscopy images

N Elmalam, A Zaritsky - bioRxiv, 2024 - biorxiv.org
In silico labeling prediction of organelle fluorescence from label-free microscopy images has
the potential to revolutionize our understanding of cells as integrated complex systems …