Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …

Byonic is the name of a software package for peptide and protein identification by tandem
mass spectrometry. This software, which has only recently become commercially available …

Biological mass spectrometry (MS) encompasses a range of methods for characterizing
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …

Monoclonal antibodies (mAbs) are powerful therapeutics, and their characterization has
drawn considerable attention and urgency. Unlike small-molecule drugs (150–600 Da) that …

Amyloid fibrils, insoluble β‐sheets structures that arise from protein misfolding, are
associated with several neurodegenerative disorders. Many small molecules have been …

The cell membrane is composed of a network of glycoconjugates including glycoproteins
and glycolipids that presents a dense matrix of carbohydrates playing critical roles in many …

Although membrane proteins are crucial participants in photosynthesis and other biological
processes, many lack high-resolution structures. Prior to achieving a high-resolution …

Fast photochemical oxidation of proteins (FPOP) is a mass spectrometry-based protein
footprinting method that modifies proteins on the microsecond time scale. Highly reactive• …

X-ray radiolytic labeling uses broadband X-rays for in situ hydroxyl radical labeling to map
protein interactions and conformation. High flux density beams are essential to overcome …