Isothermal amplification of nucleic acids
Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …
Recent advances in signal amplification strategy based on oligonucleotide and nanomaterials for microRNA detection-a review
YX Chen, KJ Huang, KX Niu - Biosensors and Bioelectronics, 2018 - Elsevier
MicroRNAs (MiRNAs) play multiple crucial regulating roles in cell which can regulate one
third of protein-coding genes. MiRNAs participate in the developmental and physiological …
third of protein-coding genes. MiRNAs participate in the developmental and physiological …
CRISPR-Cas9 Triggered Two-Step Isothermal Amplification Method for E. coli O157:H7 Detection Based on a Metal–Organic Framework Platform
X Sun, Y Wang, L Zhang, S Liu, M Zhang… - Analytical …, 2020 - ACS Publications
Escherichia coli O157: H7 has been reported as an important pathogenic bacteria causing
serious infection and economic loss. However, detection of Escherichia coli O157: H7 needs …
serious infection and economic loss. However, detection of Escherichia coli O157: H7 needs …
Bioanalytical applications of isothermal nucleic acid amplification techniques
H Deng, Z Gao - Analytica chimica acta, 2015 - Elsevier
The most popular in vitro nucleic acid amplification techniques like polymerase chain
reaction (PCR) including real-time PCR are costly and require thermocycling, rendering …
reaction (PCR) including real-time PCR are costly and require thermocycling, rendering …
Research progress on rolling circle amplification (RCA)-based biomedical sensing
Enhancing the limit of detection (LOD) is significant for crucial diseases. Cancer
development could take more than 10 years, from one mutant cell to a visible tumor. Early …
development could take more than 10 years, from one mutant cell to a visible tumor. Early …
Application of CRISPR/Cas systems in the nucleic acid detection of infectious diseases
J Li, Y Wang, B Wang, J Lou, P Ni, Y Jin, S Chen… - Diagnostics, 2022 - mdpi.com
The CRISPR/Cas system is a protective adaptive immune system against attacks from
foreign mobile genetic elements. Since the discovery of the excellent target-specific …
foreign mobile genetic elements. Since the discovery of the excellent target-specific …
An enzyme-free surface plasmon resonance biosensor for real-time detecting microRNA based on allosteric effect of mismatched catalytic hairpin assembly
J Li, P Lei, S Ding, Y Zhang, J Yang, Q Cheng… - Biosensors and …, 2016 - Elsevier
MicroRNAs (miRNAs) play significant regulatory roles in a variety of diseases and have
been emerging as a group of promising biomarkers in cancer cells. Here, a novel and …
been emerging as a group of promising biomarkers in cancer cells. Here, a novel and …
Electrochemical DNA sandwich biosensor based on enzyme amplified microRNA-21 detection and gold nanoparticles
J Mandli, H Mohammadi, A Amine - Bioelectrochemistry, 2017 - Elsevier
In this work, a novel electrochemical biosensor for miRNA-21 determination, involving a
sandwich hybridization assay onto gold nanoparticles modified pencil graphite electrode …
sandwich hybridization assay onto gold nanoparticles modified pencil graphite electrode …
One-step, ultrasensitive, and electrochemical assay of microRNAs based on T7 exonuclease assisted cyclic enzymatic amplification
Taking advantage of the special exodeoxyribonuclease activity of T7 exonuclease, a simple,
sensitive, selective, and label-free microRNA biosensor based on the cyclic enzymatic …
sensitive, selective, and label-free microRNA biosensor based on the cyclic enzymatic …
Ultrasensitive detection of cancer biomarker microRNA by amplification of fluorescence of lanthanide nanoprobes
L Lu, D Tu, Y Liu, S Zhou, W Zheng, X Chen - Nano Research, 2018 - Springer
Sensitive detection of cancer biomarker microRNAs (miRNAs) is of vital importance for
cancer diagnosis and treatment. Nonetheless, the detection sensitivity in the existing miRNA …
cancer diagnosis and treatment. Nonetheless, the detection sensitivity in the existing miRNA …