Current and new approaches in GMO detection: challenges and solutions

MA Fraiture, P Herman, I Taverniers… - BioMed research …, 2015 - Wiley Online Library
In many countries, genetically modified organisms (GMO) legislations have been
established in order to guarantee the traceability of food/feed products on the market and to …

Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products

A Holst-Jensen, B Spilsberg, AJ Arulandhu… - Analytical and …, 2016 - Springer
The emergence of high-throughput, massive or next-generation sequencing technologies
has created a completely new foundation for molecular analyses. Various selective …

Multiplex quantification of 12 European Union authorized genetically modified maize lines with droplet digital polymerase chain reaction

D Dobnik, B Spilsberg, A Bogožalec Košir… - Analytical …, 2015 - ACS Publications
Presence of genetically modified organisms (GMO) in food and feed products is regulated in
many countries. The European Union (EU) has implemented a threshold for labeling of …

Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR

AB Košir, C Divieto, J Pavšič, S Pavarelli… - Analytical and …, 2017 - Springer
Accurate and precise nucleic-acid quantification is crucial for clinical and diagnostic
decisions, as overestimation or underestimation can lead to misguided treatment of a …

[HTML][HTML] Development and applicability of a ready-to-use PCR system for GMO screening

SF Rosa, F Gatto, A Angers-Loustau, M Petrillo… - Food chemistry, 2016 - Elsevier
With the growing number of GMOs introduced to the market, testing laboratories have seen
their workload increase significantly. Ready-to-use multi-target PCR-based detection …

JRC GMO-Matrix: a web application to support Genetically Modified Organisms detection strategies

A Angers-Loustau, M Petrillo, L Bonfini, F Gatto… - BMC …, 2014 - Springer
Background The polymerase chain reaction (PCR) is the current state of the art technique for
DNA-based detection of Genetically Modified Organisms (GMOs). A typical control strategy …

A simple and accurate PCR method for detection of genetically modified rice

P Safaei, EM Aghaee, GJ Khaniki, SAK Afshari… - … of Environmental Health …, 2019 - Springer
Background Legislation regulating for labeling and use of genetically modified (GM) crops
are increased considerably worldwide in order to health and safety assurance of consumers …

Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9 …

F Debode, E Janssen, G Berben - European Food Research and …, 2013 - Springer
Abstract p35S promoter and tNOS terminator are the two primary targets for genetically
modified organism (GMO) screening. An increasing number of genetic constructions do not …

Loop-mediated isothermal amplification assays: Rapid and efficient diagnostics for genetically modified crops

M Singh, D Pal, P Sood, G Randhawa - Food control, 2019 - Elsevier
With the increase in number of globally approved genetically modified (GM) events and
diversification of traits, efficient GM detection strategies may play key role for regulatory …

DNA-based methods for detection of genetically modified events in food and supply chain

G Randhawa, M Singh, P Sood - Current science, 2016 - JSTOR
Efficient detection strategies for genetically modified (GM) crops need to be in compliance
with regulatory frameworks and address consumer concerns. The present review describes …