[HTML][HTML] Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development

T Wang, C Chen, LM Larcher, RA Barrero… - Biotechnology …, 2019 - Elsevier
Aptamers are short single-stranded nucleic acid sequences capable of binding to target
molecules in a way similar to antibodies. Due to various advantages such as prolonged …

PCR inhibitors–occurrence, properties and removal

C Schrader, A Schielke, L Ellerbroek… - Journal of applied …, 2012 - academic.oup.com
The polymerase chain reaction (PCR) is increasingly used as the standard method for
detection and characterization of microorganisms and genetic markers in a variety of sample …

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

JF Huggett, T Novak, JA Garson, C Green… - BMC research …, 2008 - Springer
Background PCR inhibition by nucleic acid extracts is a well known yet poorly described
phenomenon. Inhibition assessment generally depends on the assumption that inhibitors …

A subset of viruses thrives following microbial resuscitation during rewetting of a seasonally dry California grassland soil

AM Nicolas, ET Sieradzki, J Pett-Ridge… - Nature …, 2023 - nature.com
Viruses are abundant, ubiquitous members of soil communities that kill microbial cells, but
how they respond to perturbation of soil ecosystems is essentially unknown. Here, we …

BK polyomavirus infection and nephropathy: the virus–immune system interplay

N Babel, HD Volk, P Reinke - Nature Reviews Nephrology, 2011 - nature.com
Reactivation of latent BK polyomavirus (BKV) infection continues to be a major challenge in
renal graft recipients. Progression of BKV infection to BKV-associated nephropathy (BKVAN) …

[HTML][HTML] Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies

L El Bali, A Diman, A Bernard… - Journal of …, 2014 - ncbi.nlm.nih.gov
Human genomic DNA extracted from urine could be an interesting tool for large-scale public
health studies involving characterization of genetic variations or DNA biomarkers as a result …

Technical and methodological aspects of cell-free nucleic acids analyzes

Z Pös, O Pös, J Styk, A Mocova, L Strieskova… - International Journal of …, 2020 - mdpi.com
Analyzes of cell-free nucleic acids (cfNAs) have shown huge potential in many biomedical
applications, gradually entering several fields of research and everyday clinical care. Many …

Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds

IUH Khan, V Gannon, R Kent, W Koning… - Journal of …, 2007 - Elsevier
A real-time quantitative polymerase chain reaction (Q-PCR) assay was developed for
detecting and quantifying Escherichia coli in water samples from agricultural watersheds …

Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging

EA Falendysz, JG Lopera, F Lorenzsonn… - PLoS neglected …, 2015 - journals.plos.org
Monkeypox is a zoonosis clinically similar to smallpox in humans. Recent evidence has
shown a potential risk of increased incidence in central Africa. Despite attempts to isolate the …

Monitoring of BK viral load in renal allograft recipients by real-time PCR assays

CJ Bechert, VJ Schnadig, DA Payne… - American journal of …, 2010 - academic.oup.com
BK virus (BKV) is a nonenveloped, double-stranded DNA virus of the polyomavirus family
that primarily affects immunocompromised people. BKV may cause nephropathy in renal …