Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Multicellular organisms are composed of diverse cell types that must coordinate their
behaviors through communication. Cell–cell communication (CCC) is essential for growth …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

This book reflects the remarkable advances in time-resolved fluorescence techniques and
their role in a wide range of biological and clinical applications. Fluorescence lifetime …

The fundamental theory of Förster resonance energy transfer (FRET) was established in the
1940s. Its great power was only realized in the past 20 years after different techniques were …

The conjugation of anti-cancer drugs to endogenous ligands has proven to be an effective
strategy to enhance their pharmacological selectivity and delivery towards neoplasic tissues …

Optical imaging assays, especially fluorescence molecular assays, are minimally invasive if
not completely noninvasive, and thus an ideal technique to be applied to live specimens …

Fӧrster resonance energy transfer (FRET) has been described for more than a century.
FRET has become a mainstay for the study of protein localization in living cells and tissues …

Solution-phase and intracellular biosensing has substantially enhanced our understanding
of molecular processes foundational to biology and pathology. Optical methods are favored …

Maintaining an intact tumor environment is critical for quantitation of receptor-ligand
engagement in a targeted drug development pipeline. However, measuring receptor-ligand …