Suppression of Prolactin Secretion Partially Explains the Antidiabetic Effect of Bromocriptine in ob/ob Mice

IC Furigo, MF Suzuki, JE Oliveira… - …, 2019 - academic.oup.com
Previous studies have shown that bromocriptine mesylate (Bromo) lowers blood glucose
levels in adults with type 2 diabetes mellitus; however, the mechanism of action of the …

Harnessing the potential of bacterial oxidative folding to aid protein production

SL Slater, DAI Mavridou - Molecular Microbiology, 2021 - Wiley Online Library
Protein folding is central to both biological function and recombinant protein production. In
bacterial expression systems, which are easy to use and offer high protein yields, production …

Expression, purification and characterization of the authentic form of human growth hormone receptor antagonist G120R-hGH obtained in Escherichia coli periplasmic …

ACSC Menezes, MF Suzuki, JE Oliveira… - Protein Expression and …, 2017 - Elsevier
The human growth hormone receptor antagonist G120R-hGH precludes dimerization of GH
and prolactin receptors and consequently JAK/STAT signaling. Some modifications in this …

High production and optimization of the method for obtaining pure recombinant human prolactin

R Affonso, CR Soares, MT Ribela, P Bartolini - Protein Expression and …, 2018 - Elsevier
Prolactin is a pituitary hormone that is involved diverse physiological functions, such as
lactation, reproduction, metabolism, osmoregulation, immunoregulation, and behavior. Its …

Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography

PVS Dias, FS Arthuso, JE Oliveira, MF Suzuki… - … of Chromatography B, 2018 - Elsevier
Reversed-phase high-performance liquid chromatography (RP-HPLC) has been used to
analyze Interferon α-2 (IFN-α2) as a pure protein or as a pharmaceutical preparation: a …

Hoffmanopeltis (Diplopoda: Polydesmida), a new genus of Chelodesmidae from the Cerrado-Atlantic Forest ecotone, southeastern Brazil, with a list of species in the …

RS Bouzan, AD Brescovit, LFM Iniesta - Annales Zoologici Fennici, 2021 - BioOne
Hoffmanopeltis gen. nov.(type species Leptodesmus ravus Schubart, 1956) is described to
accommodate H. ravus (Schubart, 1956) comb. nov. and H. contiger sp. nov, from Cerrado …

Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis

FD Silva, JE Oliveira, RP Freire, MF Suzuki, CR Soares… - AMB Express, 2019 - Springer
Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions,
such as lactation, reproduction, osmotic and immune regulation. The present work describes …

[HTML][HTML] N-glycoprofiling analysis in a simple glycoprotein model: a comparison between recombinant and pituitary glycosylated human prolactin

MVN Capone, MF Suzuki, JE Oliveira, R Damiani… - Journal of …, 2015 - Elsevier
Human prolactin (hPRL) is a polypeptide hormone occurring in the non-glycosylated (NG-
hPRL) and glycosylated (G-hPRL) forms, with MM of approximately 23 and 25 kDa …

Influence of the expression vector and its elements on recombinant human prolactin synthesis in Escherichia coli; co-directional orientation of replication and …

R Affonso, MF Suzuki, GS Magalhaes… - Journal of Microbiological …, 2021 - Elsevier
The aim of the present work was to define a bacterial expression system that is particularly
efficient for the synthesis of recombinant human prolactin (hPRL). In previous work, based …

Synthesis of Human Bone Morphogenetic Protein-2 (hBMP-2) in E. coli Periplasmic Space: Its Characterization and Preclinical Testing

JE Oliveira, MF Suzuki, R Damiani, ER Lima… - Cells, 2021 - mdpi.com
Human BMP-2, a homodimeric protein that belongs to the TGF-β family, is a recognized
osteoinductor due to its capacity of inducing bone regeneration and ectopic bone formation …