Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

KP De Sousa, I Rossi, M Abdullahi… - Wiley …, 2023 - Wiley Online Library
Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer
nanoparticles secreted by most cells. EVs are regarded as important mediators of …

Extracellular vesicle quantification and characterization: common methods and emerging approaches

TA Hartjes, S Mytnyk, GW Jenster, V van Steijn… - Bioengineering, 2019 - mdpi.com
Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information
about cells by which they are secreted. Growing interest in the role of EVs in intercellular …

[HTML][HTML] A comprehensive review on recent advances in exosome isolation and characterization: Toward clinical applications

N Dilsiz - Translational Oncology, 2024 - Elsevier
Exosomes are small, round vesicles in the 30 and 120 nm diameter range released by all
living cell types. Exosomes play many essential functions in intercellular communication and …

Organ-on-chip models of cancer metastasis for future personalized medicine: From chip to the patient

D Caballero, S Kaushik, VM Correlo, JM Oliveira… - Biomaterials, 2017 - Elsevier
Most cancer patients do not die from the primary tumor but from its metastasis. Current in
vitro and in vivo cancer models are incapable of satisfactorily predicting the outcome of …

Preparation and characterization of extracellular vesicles

S Tiwari, V Kumar, S Randhawa… - American Journal of …, 2021 - Wiley Online Library
Extracellular vesicles (EVs) are heterogeneous membranous vesicles secreted by every cell
type and offer significant potential in therapy and diagnostics. Differential ultracentrifugation …

Therapeutic and diagnostic applications of extracellular vesicles

S Stremersch, SC De Smedt, K Raemdonck - Journal of Controlled Release, 2016 - Elsevier
During the past two decades, extracellular vesicles (EVs) have been identified as important
mediators of intercellular communication, enabling the functional transfer of bioactive …

The light-sheet microscopy revolution

JM Girkin, MT Carvalho - Journal of Optics, 2018 - iopscience.iop.org
This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …

Particle tracking in drug and gene delivery research: State-of-the-art applications and methods

BS Schuster, LM Ensign, DB Allan, JS Suk… - Advanced drug delivery …, 2015 - Elsevier
Particle tracking is a powerful microscopy technique to quantify the motion of individual
particles at high spatial and temporal resolution in complex fluids and biological specimens …

Applications of light-sheet microscopy in microdevices

I Albert-Smet, A Marcos-Vidal, JJ Vaquero… - Frontiers in …, 2019 - frontiersin.org
Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories
for quite some time, mainly as custom-made systems, with imaging applications ranging …

Exploiting microfluidics for extracellular vesicle isolation and characterization: potential use for standardized embryo quality assessment

B Talebjedi, N Tasnim, M Hoorfar… - Frontiers in Veterinary …, 2021 - frontiersin.org
Recent decades have seen a growing interest in the study of extracellular vesicles (EVs),
driven by their role in cellular communication, and potential as biomarkers of health and …