“Footprinting” refers to assays that examine ligand binding and conformational changes by
determining the solvent accessibility of the backbone, bases, or side chain structures of …

Mass spectrometry (MS) is increasingly being used to probe the structure and dynamics of
proteins and the complexes they form with other macromolecules. There are now several …

Specific, end-labeled DNA fragments can be simply and rapidly prepared using the
polymerase chain reaction (PCR). Such fragments are suitable for use in DNase I protection …

Abstract Effects of placing a lac operator at different positions relative to a promoter for
bacteriophage T7 RNA polymerase were tested. Transcription can be strongly repressed by …

The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is
the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact …

DNA exhibits conformational variability that is de-pendent on base composition and
environmental con-ditions. Linear DNA can adopt at least three distinct double-stranded …

Operator sequence and repressor protein regulate the activity of the lac promoter over a
greater than 1000-fold range. Combinations of the lac operator with other promoter …

Along with their cognate acyl-homoserine lactone signals, the quorum sensing regulators
LasR and RhlR control the expression of hundreds of genes in the opportunistic human …

A factor necessary for the accurate transcription of cloned Xenopus 5S genes in vitro has
been isolated from soluble extracts of X. Iaevis ovaries. The activity of the factor was …

Publisher Summary This chapter discusses that individual-site binding isotherms are
uniquely suited to permit the resolution of interaction parameters for systems exhibiting …