Genetically modified crops: detection strategies and biosafety issues

S Kamle, S Ali - Gene, 2013 - Elsevier
Genetically modified (GM) crops are increasingly gaining acceptance but concurrently
consumers' concerns are also increasing. The introduction of Bacillus thuringiensis (Bt) …

Current perspectives on genetically modified crops and detection methods

M Kamle, P Kumar, JK Patra, VK Bajpai - 3 Biotech, 2017 - Springer
Genetically modified (GM) crops are the fastest adopted commodities in the agribiotech
industry. This market penetration should provide a sustainable basis for ensuring food …

6 New Approaches to Cassava Breeding

H Ceballos, C Hershey… - Plant breeding …, 2012 - Wiley Online Library
Cassava is the third most important source of dietary energy for developing regions of the
world. It is a key staple crop and is also increasingly an important source of raw material for …

Flower color modification through co-overexpression of the VtF3′5′H and RhNHX genes in Rosa hybrida

J Xu, JY Shin, PM Park, HR An, YJ Kim, SJ Kim… - Plant Cell, Tissue and …, 2023 - Springer
Roses (Rosa hybrida) are a highly merchandised flower but lack blue varieties.
Overexpression of the flavonoid 3′, 5′-hydroxylase (F3′ 5′ H) gene can increase the …

Field performance of transgenic sugarcane produced using Agrobacterium and biolistics methods

P Joyce, S Hermann, A O'Connell… - Plant biotechnology …, 2014 - Wiley Online Library
Future genetic improvement of sugarcane depends, in part, on the ability to produce high‐
yielding transgenic cultivars with improved traits such as herbicide and insect resistance …

Transgene integration complexity and expression stability following biolistic or Agrobacterium-mediated transformation of sugarcane

H Wu, FS Awan, A Vilarinho, Q Zeng, B Kannan… - In Vitro Cellular & …, 2015 - Springer
Sugarcane (Saccharum spp. hybrids) accounts for 80% of the table sugar produced
worldwide and is also a prime feedstock for biofuel production. However, very few studies …

Assessment of Bt cotton genotypes for the Cry1Ac transgene and its expression

HMN Cheema, AA Khan, MI Khan, U Aslam… - The Journal of …, 2016 - cambridge.org
Genetically modified (GM) plants expressing Bt toxin provide protection against lepidopteran
pests. The only GM crop in Pakistan is Bt cotton, which was illegally imported and adopted …

High-throughput assessment of transgene copy number in sugarcane using real-time quantitative PCR

RE Casu, A Selivanova, JM Perroux - Plant cell reports, 2012 - Springer
Accurate and timely detection of transgene copy number in sugarcane is currently hampered
by the requirement to use Southern blotting, needing relatively large amounts of genomic …

Assessment of transgene copy number and zygosity of transgenic maize overexpressing Cry1Ie gene with SYBR® Green qRT-PCR

Y Zhang, Y Liu, J Zhang, G Wang, J Wang… - In Vitro Cellular & …, 2015 - Springer
Transgenic maize expressing the Cry1Ie gene was shown to efficiently kill susceptible and
Cry1Ac-resistant insects, indicating that Cry1Ie might be a good candidate for the …

Comparative expression analysis of reference genes in field-grown cassava

A Salcedo, C Zambrana, D Siritunga - Tropical plant biology, 2014 - Springer
Due to its accuracy, sensitivity and reproducibility, real-time PCR has emerged as a key
technique to measure changes in expression of target genes. But to obtain reliable results …