Single-particle cryo-electron microscopy (cryo-EM) is a technique that takes projection
images of biomolecules frozen at cryogenic temperatures. A major advantage of this …

Macromolecules change their shape (conformation) in the process of carrying out their
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …

Resolving the structural variability of proteins is often key to understanding the structure–
function relationship of those macromolecular machines. Single particle analysis using …

Biological macromolecules and assemblies precisely rearrange their atomic 3D structures to
execute cellular functions. Understanding the mechanisms by which these molecular …

Single particle cryo-electron microscopy (cryo-EM) has emerged as the prevailing method
for near-atomic structure determination, shedding light on the important molecular …

There is a variety of experimental and computational techniques available to explore protein
dynamics, each presenting advantages and limitations. One promising experimental …

Cryoelectron microscopy (cryo-EM) methods have made meaningful contributions in a wide
variety of scientific research fields. In structural biology, cryo-EM routinely elucidates …

ManifoldEM is an established method of geometric machine learning developed to extract
information on conformational motions of molecules from their projections obtained by …

Analyzing dynamical data often requires information of the temporal labels, but such
information is unavailable in many applications. Recovery of these temporal labels, closely …

Biomolecules undergo continuous conformational motions, a subset of which are
functionally relevant. Understanding, and ultimately controlling biomolecular function are …