The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

The widely successful use of synthetic herbicides over the past 70 years has imposed strong
and widespread selection pressure, leading to the evolution of herbicide resistance in …

Prime editing is a versatile genome-editing technology, but it suffers from low editing
efficiency. In the present study, we introduce optimized prime editors with substantially …

Targeted saturation mutagenesis of crop genes could be applied to produce genetic variants
with improved agronomic performance. However, tools for directed evolution of plant genes …

Durable crop protection is an essential component of current and future food security.
However, the effectiveness of pesticides is threatened by the evolution of resistant …

Nucleotide base editors in plants have been limited to conversion of cytosine to thymine.
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …

Base editors (BEs) have been used to create C-to-T substitutions in various organisms.
However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window …

Clustered regularly interspersed short pallindromic repeats (CRISPR) and CRISPR
associated proteins (Cas) system (CRISPR-Cas) came into light as prokaryotic defence …

Resistance to insecticides and herbicides has cost billions of US dollars in the agricultural
sector and could result in millions of lives lost to insect-vectored diseases. We mostly …

Glyphosate is the most widely used herbicide in world agriculture and for general vegetation
control in a wide range of situations. Global and often intensive glyphosate selection of very …