Metabolic glycoengineering–exploring glycosylation with bioorthogonal chemistry

M Kufleitner, LM Haiber, V Wittmann - Chemical Society Reviews, 2023 - pubs.rsc.org
Glycans are involved in numerous biological recognition events. Being secondary gene
products, their labeling by genetic methods–comparable to GFP labeling of proteins–is not …

Click chemistry for drug development and diverse chemical–biology applications

P Thirumurugan, D Matosiuk, K Jozwiak - Chemical reviews, 2013 - ACS Publications
Chemistry, traditionally being the science of synthesis and structural manipulations of
molecules, has gradually undertaken the more challenging task of biology-oriented …

Practical considerations, challenges, and limitations of bioconjugation via azide–alkyne cycloaddition

CJ Pickens, SN Johnson, MM Pressnall… - Bioconjugate …, 2017 - ACS Publications
Interrogating biological systems is often limited by access to biological probes. The
emergence of “click chemistry” has revolutionized bioconjugate chemistry by providing facile …

Cellular consequences of copper complexes used to catalyze bioorthogonal click reactions

DC Kennedy, CS McKay, MCB Legault… - Journal of the …, 2011 - ACS Publications
Copper toxicity is a critical issue in the development of copper-based catalysts for copper (I)-
catalyzed azide–alkyne cycloaddition (CuAAC) reactions for applications in living systems …

Reactivity of biarylazacyclooctynones in copper-free click chemistry

CG Gordon, JL Mackey, JC Jewett… - Journal of the …, 2012 - ACS Publications
The 1, 3-dipolar cycloaddition of cyclooctynes with azides, also called “copper-free click
chemistry”, is a bioorthogonal reaction with widespread applications in biological discovery …

In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria

N Geva-Zatorsky, D Alvarez, JE Hudak, NC Reading… - Nature medicine, 2015 - nature.com
The intestine is densely populated by anaerobic commensal bacteria. These
microorganisms shape immune system development, but understanding of host–commensal …

Glycan labeling and analysis in cells and in vivo

B Cheng, Q Tang, C Zhang… - Annual Review of …, 2021 - annualreviews.org
As one of the major types of biomacromolecules in the cell, glycans play essential functional
roles in various biological processes. Compared with proteins and nucleic acids, the …

Bringing chemistry to life

M Boyce, CR Bertozzi - Nature methods, 2011 - nature.com
Bringing chemistry to life | Nature Methods Skip to main content Thank you for visiting nature.com.
You are using a browser version with limited support for CSS. To obtain the best experience …

Ligand-directed acyl imidazole chemistry for labeling of membrane-bound proteins on live cells

S Fujishima, R Yasui, T Miki, A Ojida… - Journal of the American …, 2012 - ACS Publications
Chemistry-based protein labeling in living cells is undoubtedly useful for understanding
natural protein functions and for biological/pharmaceutical applications. Here, we report a …

CalFluors: a universal motif for fluorogenic azide probes across the visible spectrum

P Shieh, VT Dien, BJ Beahm… - Journal of the …, 2015 - ACS Publications
Fluorescent bioorthogonal smart probes across the visible spectrum will enable sensitive
visualization of metabolically labeled molecules in biological systems. Here we present a …