Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-
visible, near-IR spectral range when biological substrates are excited with light at suitable …

The function of a T cell depends on its subtype and activation state. Here, we show that
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …

Intravital microscopy (IVM) emerged and matured as a powerful tool for elucidating
pathways in biological processes. Although label-free multiphoton IVM is attractive for its …

Prokaryotic and eukaryotic cells exhibit an intrinsic natural fluorescence due to the presence
of fluorescent cellular structural components and metabolites. Therefore, cellular …

The non-invasive high-resolution spatial mapping of cell metabolism within tissues could
provide substantial advancements in assessing the efficacy of stem cell therapy and …

Monitoring subcellular functional and structural changes associated with metabolism is
essential for understanding healthy tissue development and the progression of numerous …

DNA damage signaling is critical for the maintenance of genome integrity and cell fate
decision. Poly (ADP-ribose) polymerase 1 (PARP1) is a DNA damage sensor rapidly …

Fluorescence lifetime imaging (FLI) provides unique quantitative information in biomedical
and molecular biology studies but relies on complex data-fitting techniques to derive the …

Intravital microscopy has become increasingly popular over the past few decades because it
provides high-resolution and real-time information about complex biological processes …