Solid-state NMR investigations of extracellular matrixes and cell walls of algae, bacteria, fungi, and plants

N Ghassemi, A Poulhazan, F Deligey… - Chemical …, 2021 - ACS Publications
Extracellular matrixes (ECMs), such as the cell walls and biofilms, are important for
supporting cell integrity and function and regulating intercellular communication. These …

Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

In-cell NMR: Why and how?

FX Theillet, E Luchinat - Progress in Nuclear Magnetic Resonance …, 2022 - Elsevier
NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

In-cell sensitivity-enhanced NMR of intact viable mammalian cells

R Ghosh, Y Xiao, J Kragelj… - Journal of the American …, 2021 - ACS Publications
NMR has the resolution and specificity to determine atomic-level protein structures of
isotopically labeled proteins in complex environments, and with the sensitivity gains …

Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy

S Narasimhan, C Pinto, A Lucini Paioni… - Nature protocols, 2021 - nature.com
For a long time, solid-state nuclear magnetic resonance (ssNMR) has been employed to
study complex biomolecular systems at the detailed chemical, structural, or dynamic level …

[HTML][HTML] Stability of the nitroxide biradical AMUPol in intact and lysed mammalian cells

R Ghosh, R Dumarieh, Y Xiao, KK Frederick - Journal of Magnetic …, 2022 - Elsevier
Abstract Dynamic Nuclear Polarization (DNP) enhanced solid state NMR increases
experimental sensitivity, potentially enabling detection of biomolecules at their physiological …

In-cell NMR of intact mammalian cells preserved with the cryoprotectants DMSO and glycerol have similar DNP performance

Y Xiao, R Ghosh, KK Frederick - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
NMR has the resolution and specificity to determine atomic-level protein structures of
isotopically-labeled proteins in complex environments and, with the sensitivity gains …

[HTML][HTML] In cell NMR reveals cells selectively amplify and structurally remodel amyloid fibrils

S Ansari, D Lagasca, R Dumarieh, Y Xiao, S Krishna… - …, 2024 - pmc.ncbi.nlm.nih.gov
Amyloid forms of α-synuclein adopt different conformations depending on environmental
conditions. Advances in structural biology have accelerated fibril characterization. However …

[PDF][PDF] High-resolution studies of proteins in natural membranes by solid-state NMR

D Beriashvili, RD Schellevis, F Napoli, M Weingarth… - J Vis Exp, 2021 - research-portal.uu.nl
Membrane proteins are vital for cell function and thus represent important drug targets. Solid-
state Nuclear Magnetic Resonance (ssNMR) spectroscopy offers a unique access to probe …