Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Phage therapy to treat life-threatening drug-resistant infections has been hampered by
technical challenges in phage production. Cell-free bacteriophage synthesis (CFBS) can …

Some of the most metabolically diverse species of bacteria (eg, Actinobacteria) have higher
GC content in their DNA, differ substantially in codon usage, and have distinct protein folding …

The fragility of biological systems during storage, transport, and utilization necessitates
reliable cold-chain infrastructure and limits the potential of biotechnological applications. In …

Recombinant adeno-associated viruses (rAAVs) have emerged as important tools for gene
therapy and, more recently, vaccine development. Nonetheless, manufacturing can be costly …

Recent studies have shown that the reconstituted cell-free DNA replisome and in vitro
transcription and translation systems from Escherichia coli are highly important in applied …

Cell‐free protein synthesis (CFPS) systems are an attractive method to complement the
usual cell‐based synthesis of proteins, especially for screening approaches. The literature …

The cell-free system offers potential advantages in biosensor applications, but its limited
time for protein synthesis poses a challenge in creating enough fluorescent signals to detect …

Plasmodium knowlesi is the only Plasmodium that causes zoonotic disease among the
Plasmodium that cause infection in humans. It is fatal due to its short asexual growth cycle …

The SARS-CoV-2 pandemic highlighted the urgent need for biomanufacturing paradigms
that are robust and fast. Here, we demonstrate the rapid process development and scalable …