Phagemid vectors for phage display: properties, characteristics and construction
H Qi, H Lu, HJ Qiu, V Petrenko, A Liu - Journal of molecular biology, 2012 - Elsevier
Phagemids are filamentous-phage-derived vectors containing the replication origin of a
plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural …
plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural …
MASP-3 is the exclusive pro-factor D activator in resting blood: the lectin and the alternative complement pathways are fundamentally linked
MASP-3 was discovered 15 years ago as the third mannan-binding lectin (MBL)-associated
serine protease of the complement lectin pathway. Lacking any verified substrate its role …
serine protease of the complement lectin pathway. Lacking any verified substrate its role …
Pacifastin-related peptides: structural and functional characteristics of a family of serine peptidase inhibitors
B Breugelmans, G Simonet, V van Hoef, S Van Soest… - Peptides, 2009 - Elsevier
Members of the pacifastin family are serine peptidase inhibitors, found in arthropods and
have many members within different insect orders. Based on their structural characteristics …
have many members within different insect orders. Based on their structural characteristics …
Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and-2 are essential for lectin pathway activation and reveal …
The lectin pathway is an antibody-independent activation route of the complement system. It
provides immediate defense against pathogens and altered self-cells, but it also causes …
provides immediate defense against pathogens and altered self-cells, but it also causes …
Selective inhibition of the lectin pathway of complement with phage display selected peptides against mannose-binding lectin-associated serine protease (MASP)-1 …
A Kocsis, KA Kékesi, R Szász, BM Végh… - The Journal of …, 2010 - journals.aai.org
The complement system, an essential part of the innate immune system, can be activated
through three distinct routes: the classical, the alternative, and the lectin pathways. The …
through three distinct routes: the classical, the alternative, and the lectin pathways. The …
Directed evolution reveals the binding motif preference of the LC8/DYNLL hub protein and predicts large numbers of novel binders in the human proteome
LC8 dynein light chain (DYNLL) is a eukaryotic hub protein that is thought to function as a
dimerization engine. Its interacting partners are involved in a wide range of cellular …
dimerization engine. Its interacting partners are involved in a wide range of cellular …
Arg236 in human chymotrypsin B2 (CTRB2) is a key determinant of high enzyme activity, trypsinogen degradation capacity, and protection against pancreatitis
Pancreatic chymotrypsins (CTRs) are digestive proteases that in humans include CTRB1,
CTRB2, CTRC, and CTRL. The highly similar CTRB1 and CTRB2 are the products of gene …
CTRB2, CTRC, and CTRL. The highly similar CTRB1 and CTRB2 are the products of gene …
Phage display as a powerful tool to engineer protease inhibitors
ML Zani, T Moreau - Biochimie, 2010 - Elsevier
Since its introduction by Georges Smith some 25 years ago, phage display has proved to be
a powerful molecular technique for selecting proteins with desired biological properties from …
a powerful molecular technique for selecting proteins with desired biological properties from …
Substrate specificity of human chymotrypsin-like protease (CTRL) characterized by phage display-selected small-protein inhibitors
Chymotrypsin-like protease (CTRL) is one of the four chymotrypsin isoforms expressed in
the human exocrine pancreas. Human genetic and experimental evidence indicate that …
the human exocrine pancreas. Human genetic and experimental evidence indicate that …
High affinity small protein inhibitors of human chymotrypsin C (CTRC) selected by phage display reveal unusual preference for P4′ acidic residues
Human chymotrypsin C (CTRC) is a pancreatic protease that participates in the regulation of
intestinal digestive enzyme activity. Other chymotrypsins and elastases are inactive on the …
intestinal digestive enzyme activity. Other chymotrypsins and elastases are inactive on the …