Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize
these events without interference from other regions deeper within. This review describes a …

Total internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical
technique that provides for the excitation of fluorophores in an extremely thin axial region …

Publisher Summary Total internal reflection fluorescence (TIRF) provides a means to
selectively excite just those fluorophores in an aqueous environment that are very near a …

The aggregation states of the epidermal growth factor receptor (EGFR) on single A431
human epidermoid carcinoma cells were assessed with two new techniques for determining …

Publisher Summary This chapter describes the total internal reflection fluorescence (TIRF)
microscopy in cell biology. TIRF microscopy (also called “evanescent wave microscopy”) …

Live‐cell microscopy imaging of fluorescent‐tagged fusion proteins is an essential tool for
cell biologists. Total internal reflection fluorescence microscopy (TIRFM) has joined confocal …

Total internal reflection fluorescence microscopy (TIRFM), also known as evanescent wave
microscopy, is used in a wide range of applications, particularly to view single molecules …

Biological research has always tremendously benefited from the development of key
methodology. In fact, it was the advent of microscopy that shaped our understanding of cells …

During metastasis to distant sites, tumor cells migrate to blood vessels. In vivo, breast tumor
cells utilize a specialized mode of migration known as streaming, where a linear assembly of …

The distribution and dynamics of molecules at or near surfaces are central to numerous
phenomena in biology: for example, binding to and triggering of cells by hormones …