Top-down proteomics and the challenges of true proteoform characterization
A Po, CE Eyers - Journal of proteome research, 2023 - ACS Publications
Top-down proteomics (TDP) aims to identify and profile intact protein forms (proteoforms)
extracted from biological samples. True proteoform characterization requires that both the …
extracted from biological samples. True proteoform characterization requires that both the …
Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications
Proteoforms are all forms of protein molecules from the same gene because of variations at
the DNA, RNA, and protein levels, eg, alternative splicing and post-translational …
the DNA, RNA, and protein levels, eg, alternative splicing and post-translational …
Defining proteoform-specific interactions for drug targeting in a native cell signalling environment
CA Lutomski, JL Bennett, TJ El-Baba, D Wu… - Nature Chemistry, 2025 - nature.com
Understanding the dynamics of membrane protein–ligand interactions within a native lipid
bilayer is a major goal for drug discovery. Typically, cell-based assays are used, however …
bilayer is a major goal for drug discovery. Typically, cell-based assays are used, however …
Small-Scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-Down Proteomics of Large Proteoforms
Top-down-mass spectrometry (MS)-based proteomics has emerged as a premier technology
to examine proteins at the proteoform level, enabling characterization of genetic mutations …
to examine proteins at the proteoform level, enabling characterization of genetic mutations …
Top-down proteomics of myosin light chain isoforms define chamber-specific expression in the human heart
Myosin functions as the “molecular motor” of the sarcomere and generates the contractile
force necessary for cardiac muscle contraction. Myosin light chains 1 and 2 (MLC-1 and-2) …
force necessary for cardiac muscle contraction. Myosin light chains 1 and 2 (MLC-1 and-2) …
Comprehensive characterization of endogenous phospholamban proteoforms enabled by photocleavable surfactant and top-down proteomics
Top-down mass spectrometry (MS)-based proteomics has become a powerful tool for
analyzing intact proteins and their associated post-translational modifications (PTMs). In …
analyzing intact proteins and their associated post-translational modifications (PTMs). In …
Top-down proteomics
Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
Recent progress in quantitative phosphoproteomics
K Zittlau, P Nashier, C Cavarischia-Rega… - Expert Review of …, 2023 - Taylor & Francis
Introduction Protein phosphorylation is a critical post-translational modification involved in
the regulation of numerous cellular processes from signal transduction to modulation of …
the regulation of numerous cellular processes from signal transduction to modulation of …
The 2023 report on the proteome from the HUPO human proteome project
Since 2010, the Human Proteome Project (HPP), the flagship initiative of the Human
Proteome Organization (HUPO), has pursued two goals:(1) to credibly identify the protein …
Proteome Organization (HUPO), has pursued two goals:(1) to credibly identify the protein …
Improved characterization of trastuzumab deruxtecan with PTCR and internal fragments implemented in middle-down MS workflows
C Beaumal, E Deslignière, H Diemer… - Analytical and …, 2024 - Springer
Antibody-drug conjugates (ADCs) are highly complex proteins mainly due to the structural
microvariability of the mAb, along with the additional heterogeneity afforded by the …
microvariability of the mAb, along with the additional heterogeneity afforded by the …