Advanced strategies in high-throughput droplet screening for enzyme engineering

W Yan, X Li, D Zhao, M Xie, T Li, L Qian, C Ye… - Biosensors and …, 2024 - Elsevier
Enzymes, as biocatalysts, play a cumulatively important role in environmental purification
and industrial production of chemicals and pharmaceuticals. However, natural enzymes are …

Artificial Polymerizations in Living Organisms for Biomedical Applications

Y Chen, BSN Tan, Y Cheng… - Angewandte Chemie …, 2024 - Wiley Online Library
Within living organisms, numerous nanomachines are constantly involved in complex
polymerization processes, generating a diverse array of biomacromolecules for maintaining …

Functionalized polysaccharides improve sensitivity of tyramide/peroxidase proximity labeling assays through electrostatic interactions

M Heiniger, R Vanella, Z Walsh-Korb… - … Biomaterials Science & …, 2024 - ACS Publications
High-throughput assays that efficiently link genotype and phenotype with high fidelity are
key to successful enzyme engineering campaigns. Among these assays, the …

Use of Microfluidics for Directed Evolution of Biocatalysts and Materials in Droplets and Microgels

NP Kokar, G Vladisavljević, R Ostafe, R Prodanović - 2024 - preprints.org
Microfluidics is used for droplet-based biochemical and biological assays in protein and
metabolic engineering for designing novel biocatalysts. These systems are based on lab-on …

Understanding activity-stability tradeoffs in biocatalysts by enzyme proximity sequencing

R Vanella, C Küng, AA Schoepfer, V Doffini… - Nature …, 2024 - nature.com
Understanding the complex relationships between enzyme sequence, folding stability and
catalytic activity is crucial for applications in industry and biomedicine. However, current …