[HTML][HTML] Engineering artificial cross-species promoters with different transcriptional strengths
W Zuo, G Yin, L Zhang, W Zhang, R Xu, Y Wang… - Synthetic and Systems …, 2025 - Elsevier
As a fundamental tool in synthetic biology, promoters are pivotal in regulating gene
expression, enabling precise genetic control and spurring innovation across diverse …
expression, enabling precise genetic control and spurring innovation across diverse …
Novel His-tag Variants for Insertion Inside Polypeptide Chain
AG Tarabarova, A Lopukhov, AN Fedorov… - ACS …, 2023 - ACS Publications
His-tags are protein affinity tags ubiquitously used due to their convenience and
effectiveness. However, in some individual cases, the attachment of His-tags to a protein's N …
effectiveness. However, in some individual cases, the attachment of His-tags to a protein's N …
Hotspot Wizard‐informed engineering of a hyperthermophilic β‐glucosidase for enhanced enzyme activity at low temperatures
ME Erkanli, K El‐Halabi, TK Kang… - Biotechnology and …, 2024 - Wiley Online Library
Hyperthermophilic enzymes serve as an important source of industrial enzymes due to their
high thermostability. Unfortunately, most hyperthermophilic enzymes suffer from reduced …
high thermostability. Unfortunately, most hyperthermophilic enzymes suffer from reduced …
Characterization and structural analysis of a leucine aminopeptidase using site-directed mutagenesis
Y Men, Y Liu, D Yin, G Wang, R Qin, H Xiong, Y Wang - AMB Express, 2024 - Springer
Abstract Amp0279 (EC 3.4. 11.24, GenBank: CP000817. 1) is a Co 2+-dependent leucine
aminopeptidase from the Lysinibacillus sphaericus C3-41 genome. After analyses using …
aminopeptidase from the Lysinibacillus sphaericus C3-41 genome. After analyses using …
His-Tag Effect on Biochemical Properties of B. Subtilis US572 α-Amylase Produced in E. coli: Application of the Recombinant Enzyme in Breadmaking
K Salem, F Elgharbi, H Ben Hlima… - Food …, 2024 - Taylor & Francis
The gene encoding Bacillus subtilis α-amylase was cloned into pET-21a (+). The expression
level of the recombinant enzyme was 10.7-fold higher than the expression level of the native …
level of the recombinant enzyme was 10.7-fold higher than the expression level of the native …