Preparation of Staphylococcus aureus genomic DNA using a chloroform extraction and ethanol precipitation method, followed by additional cleanup and quantification …

MS Zeden, A Gründling - Cold Spring Harbor Protocols, 2023 - cshprotocols.cshlp.org
In this protocol, we describe the isolation of genomic DNA (gDNA) from Staphylococcus
aureus strains using a chloroform extraction and ethanol precipitation method. This gDNA …

Preparation of Staphylococcus aureus genomic DNA using Promega Nuclei Lysis and Protein Precipitation Solutions, followed by additional cleanup and …

MS Zeden, A Gründling - Cold Spring Harbor Protocols, 2023 - cshprotocols.cshlp.org
In this protocol, we describe the isolation of genomic DNA (gDNA) from Staphylococcus
aureus using the Promega Nuclei Lysis and Protein Precipitation solutions. Gram-positive …

Single cell RNA-seq: a novel tool to unravel virus-host interplay

HR Jogi, N Smaraki, SS Nayak, D Rajawat, DJ Kamothi… - VirusDisease, 2024 - Springer
Over the last decade, single cell RNA sequencing (scRNA-seq) technology has caught the
momentum of being a vital revolutionary tool to unfold cellular heterogeneity by high …

Selection of antibiotic-resistant bacterial strains and identification of genomic alterations by whole-genome sequencing: using Staphylococcus aureus and oxacillin …

MS Zeden, A Gründling - Cold Spring Harbor Protocols, 2023 - cshprotocols.cshlp.org
Here, we discuss methods for the selection of antibiotic-resistant bacteria and the use of
high-throughput whole-genome sequencing for the identification of the underlying …

Allelic-exchange procedure in Staphylococcus aureus

MS Zeden, CF Schuster… - Cold Spring Harbor …, 2023 - cshprotocols.cshlp.org
This protocol continues a series of methods for the construction of an in-frame gene deletion
in Staphylococcus aureus strain RN4220. To this end, we describe in this protocol an allelic …