When used appropriately, a confocal fluorescence microscope is an excellent tool for
making quantitative measurements in cells and tissues. The confocal microscope's ability to …

After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

The interaction of regulatory proteins with the complex nucleoprotein structures that are
found in mammalian cells involves chromatin reorganization at multiple levels. Mechanisms …

Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy
with a tremendous potential for impacting many fields of science. Several super-resolution …

Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …

This protocol outlines a procedure for collecting and analyzing point spread functions
(PSFs). It describes how to prepare fluorescent microsphere samples, set up a confocal …

Imaging of living cells and tissue is now common in many fields of the life and physical
sciences, and is instrumental in revealing a great deal about cellular dynamics and function …

Synthesis of mRNA in eukaryotes involves the coordinated action of many enzymatic
processes, including initiation, elongation, splicing, and cleavage. Kinetic competition …

Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new
single-photon (SP) array detectors, which give access to an entirely new set of single-photon …

Molecular diffusion and transport processes are fundamental in physical, chemical, and
biological systems. Current approaches to measuring molecular transport in cells and …