Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

L Yao, K Shabestary, SM Björk… - Nature …, 2020 - nature.com
Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology
applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we …

Regulatory RNA at the crossroads of carbon and nitrogen metabolism in photosynthetic cyanobacteria

AM Muro-Pastor, WR Hess - Biochimica et Biophysica Acta (BBA)-Gene …, 2020 - Elsevier
Cyanobacteria are photosynthetic bacteria that populate widely different habitats.
Accordingly, cyanobacteria exhibit a wide spectrum of lifestyles, physiologies, and …

Generation of synthetic shuttle vectors enabling modular genetic engineering of cyanobacteria

F Opel, NA Siebert, S Klatt, A Tüllinghoff… - ACS synthetic …, 2022 - ACS Publications
Cyanobacteria have raised great interest in biotechnology due to their potential for a
sustainable, photosynthesis-driven production of fuels and value-added chemicals. This has …

Analysis of a photosynthetic cyanobacterium rich in internal membrane systems via gradient profiling by sequencing (Grad-seq)

M Riediger, P Spät, R Bilger, K Voigt, B Maček… - The Plant …, 2021 - academic.oup.com
Although regulatory small RNAs have been reported in photosynthetic cyanobacteria, the
lack of clear RNA chaperones involved in their regulation poses a conundrum. Here, we …

Cyanobacterial extracellular polymeric substances (EPS)

R Mota, C Flores, P Tamagnini - Polysaccharides of microbial origin …, 2022 - Springer
Cyanobacteria are a very particular group of photoautotrophic prokaryotic organisms with
major ecological significance in the carbon and nitrogen cycles. Most cyanobacterial strains …

Thioredoxin A regulates protein synthesis to maintain carbon and nitrogen partitioning in cyanobacteria

MJ Mallén-Ponce, FJ Florencio, MJ Huertas - Plant Physiology, 2024 - academic.oup.com
Thioredoxins play an essential role in regulating enzyme activity in response to
environmental changes, especially in photosynthetic organisms. They are crucial for …

Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria

L Hemm, A Miucci, A Kraus, M Riediger, S Tholen… - RNA biology, 2024 - Taylor & Francis
Throughout the tree of life RNA-binding proteins play important roles, but they are poorly
characterized in cyanobacteria. Overexpression of the predicted RNA-binding protein …

Transcriptome-wide in vivo mapping of cleavage sites for the compact cyanobacterial ribonuclease E reveals insights into its function and substrate recognition

UA Hoffmann, F Heyl, SN Rogh, T Wallner… - Nucleic acids …, 2021 - academic.oup.com
Ribonucleases are crucial enzymes in RNA metabolism and post-transcriptional regulatory
processes in bacteria. Cyanobacteria encode the two essential ribonucleases RNase E and …

6S RNA plays a role in recovery from nitrogen depletion in Synechocystis sp. PCC 6803

B Heilmann, K Hakkila, J Georg, T Tyystjärvi… - BMC microbiology, 2017 - Springer
Abstract Background The 6S RNA is a global transcriptional riboregulator, which is
exceptionally widespread among most bacterial phyla. While its role is well-characterized in …

Glycosyltransferase Slr1064 regulates carbon metabolism by modulating the levels of UDP‐GlcNAc in Synechocystis sp. PCC 6803

Y Han, H Ge, C Xu, G Zeng, Z Li, X Huang… - New …, 2024 - Wiley Online Library
Glycosyltransferases (GTs) are enzymes that transfer sugars to various targets. They play
important roles in diverse biological processes, including photosynthesis, cell motility …