Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically
relevant cell–cell and cell–matrix interactions, gene expression and signaling pathway …

Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …

Biological specimens are intrinsically three dimensional; however, because of the obscuring
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …

The complex interaction of cells with biomaterials (ie, materiobiology) plays an increasingly
pivotal role in the development of novel implants, biomedical devices, and tissue …

Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields
have been combined only recently to facilitate novel experiments and measurements in …

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

We report a three-dimensional microscopy technique—swept, confocally-aligned planar
excitation (SCAPE) microscopy—that allows volumetric imaging of living samples at …

Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that
consist of many unaligned three-dimensional image tiles, which must be reconstructed …