DNA barcoding has been widely used for herb identification in recent decades, enabling
safety and innovation in the field of herbal medicine. In this article, we summarize recent …

High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and
sequence matching. The melting profile of a PCR product depends on its GC content, length …

Transition of the double‐stranded DNA molecule to its two single strands, DNA denaturation
or melting, has been used for many years to study DNA structure and composition. Recent …

Recent advances in fluorescent dyes, methods, instruments and software for DNA melting
analysis have created versatile new tools for variant scanning and genotyping. High …

The importance of real-time polymerase chain reaction (PCR) has increased steadily in
clinical applications over the last decade. Many applications utilize SYBR Green I dye to …

High resolution melting (HRM) is a convenient method for gene scanning as well as
genotyping of individual and multiple single nucleotide polymorphisms (SNPs). This rapid …

Pharmaceutical analysis is a discipline based on chemical, physical, biological, and
information technologies. At present, biotechnological analysis is a short branch in …

Separation of the two strands of DNA with heat (melting) is a fundamental property of DNA
that is conveniently monitored with fluorescence. Conventional melting is performed after …

This protocol permits the simultaneous mutation scanning and genotyping of PCR products
by high-resolution DNA melting analysis. This is achieved using asymmetric PCR performed …

Background The development of targeted therapies has created a pressing clinical need for
the rapid and robust molecular characterisation of cancers. We describe here the application …