During development, the genetic content of each cell remains, with a few exceptions,
identical to that of the zygote. Differentiated cells, therefore, retain all the genetic information …

It has now been 13 years since the first cloned mammal Dolly the sheep was generated from
somatic cells using nuclear transfer (SCNT). Since then, this technique has been considered …

Nuclear transfer stem cells hold considerable promise in the field of regenerative medicine
and cell-based drug discovery. In this study, a total of 29 oocytes were obtained from three …

Recently, ES cell lines were established from single blastomeres taken from eight-cell
embryos in mice and humans with success rates of 4% and 2%, respectively, which …

Although it has now been 10 years since the first cloned mammals were generated from
somatic cells using nuclear transfer (NT), most cloned embryos usually undergo …

Somatic cell nuclear transfer (SCNT) has shown tremendous potential for understanding the
mechanisms of reprogramming and creating applications in the realms of agriculture …

In mammalian cloning, evidence suggests that genomic reprogramming factors are located
in the nucleus rather than the cytoplasm of oocytes or zygotes. However, little is known …

To clarify the causes of the poor success rate of somatic cell nuclear transfer (SCNT), we
addressed the impact of abnormalities observed at early cleavage stages of development on …

Which comes first: tissue structure or cell differentiation? Although different cell types
establish distinct structures delineating the inside and outside of an embryo, they …

High rates of embryonic, fetal, or placental abnormalities have consistently been observed in
bovine cloning. Segregation of inner cell mass (ICM) and trophectoderm (TE) lineages in …