Evolution of the probe-based loop-mediated isothermal amplification (LAMP) assays in pathogen detection

X Zhang, Y Zhao, Y Zeng, C Zhang - Diagnostics, 2023 - mdpi.com
Loop-mediated isothermal amplification (LAMP), as the rank one alternative to a polymerase
chain reaction (PCR), has been widely applied in point-of-care testing (POCT) due to its …

Microfluidic-based virus detection methods for respiratory diseases

EA Tarim, B Karakuzu, C Oksuz, O Sarigil… - Emergent …, 2021 - Springer
With the recent SARS-CoV-2 outbreak, the importance of rapid and direct detection of
respiratory disease viruses has been well recognized. The detection of these viruses with …

Detecting amplicons of loop‐mediated isothermal amplification

K Shirato - Microbiology and immunology, 2019 - Wiley Online Library
Loop‐mediated isothermal amplification (LAMP) assays are used to detect diverse
pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real‐time …

Propagation of rhinovirus C in differentiated immortalized human airway HBEC3-KT epithelial cells

M Nakauchi, N Nagata, I Takayama, S Saito, H Kubo… - Viruses, 2019 - mdpi.com
Rhinoviruses (RVs) are classified into three species: RV-A, B, and C. Unlike RV-A and-B, RV-
C cannot be propagated using standard cell culture systems. In order to isolate RV-Cs from …

Evidence of the simultaneous replications of active viruses in specimens positive for multiple respiratory viruses

M Kawase, R Suwa, S Sugimoto, M Kakizaki… - Microbiology …, 2024 - Am Soc Microbiol
Genetic diagnostic assays for the detection of respiratory viruses sometimes show
simultaneous multiple infections with low copy numbers. In such cases, the disease is …

Rapid detection of an I38T amino acid substitution in influenza polymerase acidic subunit associated with reduced susceptibility to baloxavir marboxil

M Nakauchi, E Takashita, S Fujisaki… - Influenza and other …, 2020 - Wiley Online Library
Background The novel cap‐dependent endonuclease inhibitor baloxavir marboxil was
approved in February 2018 for the treatment of influenza virus infection in Japan. In vitro …

[HTML][HTML] Development of a real-time fluorescent reverse transcription loop-mediated isothermal amplification assay with quenching primers for rapid detection of …

K Okamoto, K Kadosawa, R Suzuki, E Aonuma… - Journal of Virological …, 2024 - Elsevier
Rubella virus infection during early pregnancy sometimes causes severe birth defects
termed congenital rubella syndrome. Although there are safe and effective live-attenuated …

[HTML][HTML] Loop-mediated isothermal amplification (LAMP) for detection of atypical enterovirus D68 strain VR-1197

AK Tveten, HL Ørstenvik, I Tolaas - Journal of Virological Methods, 2024 - Elsevier
A method that has rapidly evolved for detection of viral pathogens are loop-mediated
isothermal amplification (LAMP) assays. The available LAMP assays usually target the most …

Clinical evaluation of fully automated molecular diagnostic system “Simprova” for influenza virus, respiratory syncytial virus, and human metapneumovirus

I Takayama, S Semba, K Yokono, S Saito… - Scientific Reports, 2020 - nature.com
Influenza virus, respiratory syncytial virus, and human metapneumovirus commonly cause
acute upper and lower respiratory tract infections, especially in children and the elderly …

Diagnostic Potential of Rhinovirus C using Reverse-Transcription Loop-mediated Isothermal Amplification (RT-LAMP)

YP Wong, KK Tee, HY Chee - 2024 - researchsquare.com
Abstract Rhinoviruses (RVs), particularly RV-C, frequently cause acute respiratory infections
and asthma exacerbations. However, there is a lack of routine detection methods. Thus, this …