Next-Gen Dual Transcriptomics for Adult Extrapulmonary Tuberculosis Biomarkers and Host–Pathogen Interplay in Human Cells: A Strategic Review

M Nesakumar, EH Luke, U Vetrivel - Indian Journal of Microbiology, 2024 - Springer
Tuberculosis (TB) is a major public health concern that results in significant morbidity and
mortality, particularly in middle-to low-income countries. Extra-pulmonary tuberculosis …

Subgingival host–microbiome metatranscriptomic changes following scaling and root planing in grade II/III periodontitis

AE Duran‐Pinedo, J Solbiati, F Teles… - Journal of Clinical …, 2023 - Wiley Online Library
Aim To assess the effects of scaling and root planing (SRP) on the dynamics of gene
expression by the host and the microbiome in subgingival plaque samples. Materials and …

An overview of next generation sequencing strategies and genomics tools used for tuberculosis research

S Deb, J Basu, M Choudhary - Journal of Applied Microbiology, 2024 - academic.oup.com
Tuberculosis (TB) is a grave public health concern and is considered the foremost
contributor to human mortality resulting from infectious disease. Due to the stringent clonality …

Dual RNA-seq reveals a type 6 secretion system-dependent blockage of TNF-α signaling and BicA as a Burkholderia pseudomallei virulence factor important during …

JI Sanchez-Villamil, D Tapia, N Khakhum… - Gut …, 2022 - Taylor & Francis
Melioidosis is a disease caused by the Gram-negative bacillus Burkholderia pseudomallei
(Bpm), commonly found in soil and water of endemic areas. Naturally acquired human …

The functional response of human monocyte-derived macrophages to serum amyloid A and Mycobacterium tuberculosis infection

M Kawka, R Płocińska, P Płociński… - Frontiers in …, 2023 - frontiersin.org
Introduction In the course of tuberculosis (TB), the level of major acute phase protein,
namely serum amyloid A (hSAA-1), increases up to a hundredfold in the pleural fluids of …

An approach to analyze spatiotemporal patterns of gene expression at single-cell resolution in Candida albicans-infected mouse tongues

E Lindemann-Perez, DL Rodríguez, JC Pérez - Msphere, 2024 - journals.asm.org
Microbial gene expression measurements derived from infected organs are invaluable to
understand pathogenesis. However, current methods are limited to “bulk” analyses that …

Single‐cell RNA sequencing integrated with bulk RNA sequencing analysis reveals the protective effects of lactate‐mediated lactylation of microglia‐related proteins …

B Zhang, F Li, Y Shi, C Ji, Q Kong… - CNS Neuroscience & …, 2024 - Wiley Online Library
Abstract Background and Objectives Spinal cord injury (SCI) results in significant
neurological deficits, and microglia play the critical role in regulating the immune …

Clinical isolates of Mycobacterium tuberculosis with different genotypes exhibit distinct host macrophage responses in vitro

C Liang, X Li, Q Li, X Zhang, W Ren… - Journal of Medical …, 2022 - microbiologyresearch.org
Introduction. Mycobacterium tuberculosis (M. tb), the causative agent of tuberculosis, can
survive as an intracellular parasite after entering macrophages via phagocytosis. M. tb …

Mycobacterium tuberculosis-THP-1 like macrophages protein-protein interaction map revealed through dual RNA-seq analysis and a computational approach

C Hkimi, S Kamoun, O Khamessi… - Journal of Medical …, 2024 - microbiologyresearch.org
Introduction. Infection caused by Mycobacterium tuberculosis (M. tb) is still a leading cause
of mortality worldwide with estimated 1.4 million deaths annually. Hypothesis/Gap statement …

Developing new tools to fight human pathogens: A journey through the advances in RNA technologies

VG Costa, SM Costa, M Saramago, MV Cunha… - Microorganisms, 2022 - mdpi.com
A long scientific journey has led to prominent technological advances in the RNA field, and
several new types of molecules have been discovered, from non-coding RNAs (ncRNAs) to …