Characterization of proteomes aims to comprehensively characterize proteins in cells or
tissues via two main strategies:(1) bottom-up strategy based on the separation and …

This work demonstrates the utility of high-throughput nanoLC-MS and label-free
quantification (LFQ) for sample-limited bottom-up proteomics analysis, including single-cell …

In this work, we describe the construction and application of a repurposed 3D-printer as a
fraction collector. We utilize a nano-LC to ensure minimal volumes and surfaces although …

COS-7, CV-1 in origin with SV40 genes; DDA, data-dependent acquisition; DIA,
dataindependent acquisition; FAIMS, high-field asymmetric waveform ion mobility …

单细胞多组学技术为生命科学和医学注入新的活力, 能够从更微观的细胞尺度,
更高维的时空角度, 更交叉的组学视野来探索生命的未知, 拓展医学的边界 …

In this work we describe a low loss fractionation system comprised of a reconfigured Evosep
One LC system for the first dimension and a repurposed 3D-printer as a fraction collector …

Recent advances have allowed for the detection of 10,000 proteins from cultured human cell
samples, such as HeLa and HEK293T cells in a single-shot proteome analysis. However …

Despite the high prevalence of alcoholic liver disease, its identification and characterization
remain poor, especially in early stages such as alcoholic fatty liver disease and alcoholic …