Demystifying emerging bulk RNA-Seq applications: the application and utility of bioinformatic methodology

AS Thind, I Monga, PK Thakur, P Kumari… - Briefings in …, 2021 - academic.oup.com
Significant innovations in next-generation sequencing techniques and bioinformatics tools
have impacted our appreciation and understanding of RNA. Practical RNA sequencing …

Contamination issue in viral metagenomics: problems, solutions, and clinical perspectives

H Jurasz, T Pawłowski, K Perlejewski - Frontiers in Microbiology, 2021 - frontiersin.org
We describe the most common internal and external sources and types of contamination
encountered in viral metagenomic studies and discuss their negative impact on sequencing …

[HTML][HTML] Next-generation sequencing for constitutional variants in the clinical laboratory, 2021 revision: a technical standard of the American College of Medical …

C Rehder, LJH Bean, D Bick, E Chao, W Chung… - Genetics in …, 2021 - Elsevier
Next-generation sequencing (NGS) technologies are now established in clinical laboratories
as a primary testing modality in genomic medicine. These technologies have reduced the …

The human “contaminome”: bacterial, viral, and computational contamination in whole genome sequences from 1000 families

B Chrisman, C He, JY Jung, N Stockham, K Paskov… - Scientific Reports, 2022 - nature.com
The unmapped readspace of whole genome sequencing data tends to be large but is often
ignored. We posit that it contains valuable signals of both human infection and …

Next-generation sequencing applications for the study of fungal pathogens

S Jiang, Y Chen, S Han, L Lv, L Li - Microorganisms, 2022 - mdpi.com
Next-generation sequencing (NGS) has become a widely used technology in biological
research. NGS applications for clinical pathogen detection have become vital technologies …

Benchmarking machine learning robustness in COVID-19 genome sequence classification

S Ali, B Sahoo, A Zelikovsky, PY Chen, M Patterson - Scientific Reports, 2023 - nature.com
The rapid spread of the COVID-19 pandemic has resulted in an unprecedented amount of
sequence data of the SARS-CoV-2 genome—millions of sequences and counting. This …

Technical and methodological aspects of cell-free nucleic acids analyzes

Z Pös, O Pös, J Styk, A Mocova, L Strieskova… - International Journal of …, 2020 - mdpi.com
Analyzes of cell-free nucleic acids (cfNAs) have shown huge potential in many biomedical
applications, gradually entering several fields of research and everyday clinical care. Many …

Toward a Predictive Understanding of Cyanobacterial Harmful Algal Blooms through AI Integration of Physical, Chemical, and Biological Data

BL Marrone, S Banerjee, A Talapatra… - ACS Es&t …, 2023 - ACS Publications
Freshwater cyanobacterial harmful algal blooms (cyanoHABs) are a worldwide problem
resulting in substantial economic losses, due to harm to drinking water supplies, commercial …

De novo identification of microbial contaminants in low microbial biomass microbiomes with Squeegee

Y Liu, RAL Elworth, MD Jochum, KM Aagaard… - Nature …, 2022 - nature.com
Computational analysis of host-associated microbiomes has opened the door to numerous
discoveries relevant to human health and disease. However, contaminant sequences in …

Host-Parasite Interaction of Atlantic salmon (Salmo salar) and the Ectoparasite Neoparamoeba perurans in Amoebic Gill Disease

NA Botwright, AR Mohamed, J Slinger… - Frontiers in …, 2021 - frontiersin.org
Marine farmed Atlantic salmon (Salmo salar) are susceptible to recurrent amoebic gill
disease (AGD) caused by the ectoparasite Neoparamoeba perurans over the growout …