Cell‐type‐aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus
In plants, the biosynthetic pathways of some specialized metabolites are partitioned into
specialized or rare cell types, as exemplified by the monoterpenoid indole alkaloid (MIA) …
specialized or rare cell types, as exemplified by the monoterpenoid indole alkaloid (MIA) …
A protoplast-based transient gene expression assay for the identification of heat and oxidative stress-regulatory genes in perennial ryegrass
S Lei, Y Zhu, W Jia, J Zhang, Y Chi, B Xu - Plant Methods, 2024 - Springer
Background With the accumulating omics data, an efficient and time-saving transient assay
to express target genes is desired. Mesophyll protoplasts, maintaining most stress …
to express target genes is desired. Mesophyll protoplasts, maintaining most stress …
Critical parameters for robust Agrobacterium‐mediated transient transformation and quantitative promoter assays in Catharanthus roseus seedlings
LF Cole‐Osborn, E Meehan, CWT Lee‐Parsons - Plant Direct, 2024 - Wiley Online Library
Agrobacterium‐mediated transient expression methods are widely used to study gene
function in both model and non‐model plants. Using a dual‐luciferase assay, we quantified …
function in both model and non‐model plants. Using a dual‐luciferase assay, we quantified …
Transient gene expression in petunia flower petals as a toolbox
H Duan - The Journal of Horticultural Science and Biotechnology, 2024 - Taylor & Francis
Agroinfiltration-mediated transient expression is widely used in plant gene functional
studies. In this study, the functionality of a metabolic engineering construct containing …
studies. In this study, the functionality of a metabolic engineering construct containing …
PRIDA, a protoplast-based assay for efficient identification of oxidative-and heat-stress regulatory genes
S Lei, L Zeng, W Jia, J Zhang, Y Chi, B Xu - 2023 - researchsquare.com
Background: Rapidly identifying candidate genes potentially involved in stress tolerance is
crucial for functional genetic studies in non-model plant species. Agrobacterium-based …
crucial for functional genetic studies in non-model plant species. Agrobacterium-based …