Cryopreservation techniques for ram sperm

A Saha, M Asaduzzaman… - Veterinary medicine …, 2022 - Wiley Online Library
Germplasm storage and transportation in artificial insemination (AI) and other advanced
technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of …

Effect of different extenders and storage temperatures on sperm viability of liquid ram semen

H Paulenz, L Söderquist, R Pérez-Pé, KA Berg - Theriogenology, 2002 - Elsevier
Semen was collected with an artificial vagina from four adult rams. The ejaculates were
pooled and diluted, using a split-sample technique, in four different extenders: one for milk …

[HTML][HTML] Factors affecting the success of laparoscopic artificial insemination in sheep

EA Spanner, SP de Graaf, JP Rickard - Animal Reproduction Science, 2024 - Elsevier
Successful artificial breeding underpins rapid genetic and production gains in animal
agriculture. In sheep, artificial insemination with frozen semen is performed via intrauterine …

Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose-based hypertonic extenders

E Aisen, M Quintana, V Medina, H Morello, A Venturino - Cryobiology, 2005 - Elsevier
The ability of a range of extenders to cryopreserve ram spermatozoa was tested. The
extenders were modified by the inclusion of citrate, Tris buffer, trehalose, and EDTA …

Effect of the cryopreservation process on the activity and immunolocalization of antioxidant enzymes in ram spermatozoa

E Marti, JI Marti, T Muiño‐Blanco… - Journal of …, 2008 - Wiley Online Library
In this study, certain enzymes in ram semen involved in reactive oxygen species elimination
and their changes during the cryopreservation process were characterized in order to …

Successful ram semen cryopreservation with lyophilized egg yolk-based extender

S Alcay, MB Toker, E Gokce, B Ustuner, NT Onder… - Cryobiology, 2015 - Elsevier
The aim of this study was to evaluate the effects of lyophilized egg yolk extender on ram
semen cryopreservation. Ejaculates with a thick consistency, rapid wave motion (3–5 on a 0 …

Evaluation of plasma membrane integrity of frozen–thawed goat spermatozoa with or without seminal plasma

GA Azeredo, CR Esper, KT Resende - Small Ruminant Research, 2001 - Elsevier
The aim of the present work was to evaluate plasma membrane integrity, motility, vigor and
morphology of fresh and frozen goat spermatozoa with or without seminal plasma. Semen …

Effect of freeze–thawing process on lipid peroxidation, miRNAs, ion channels, apoptosis and global DNA methylation in ram spermatozoa

İH Güngör, A Tektemur, G Arkali… - Reproduction …, 2021 - CSIRO Publishing
This study was carried out to investigate the effect of the semen freeze–thawing process on
the functionality and molecular structure of ram spermatozoa. The temperature of pooled …

Effect of thawing rates and antioxidants on semen cryopreservation in Hu sheep

C Wu, J Dai, S Zhang, L Sun, Y Liu… - Biopreservation and …, 2021 - liebertpub.com
Hu sheep is a valuable sheep breed in China, and semen cryopreservation of Hu sheep is
important for sustainable development of the agri-food industry. This study aimed to evaluate …

Frequency of semen collection affects ram sperm cryoresistance

C Palacin-Martinez, M Alvarez, R Montes-Garrido… - Animals, 2022 - mdpi.com
Simple Summary Sperm cryopreservation is an important step to optimize artificial
insemination protocols for rams. Traditionally, the improvement of ram sperm …