Cryopreservation as a key element in the successful delivery of cell-based therapies—a review

J Meneghel, P Kilbride, GJ Morris - Frontiers in medicine, 2020 - frontiersin.org
Cryopreservation is a key enabling technology in regenerative medicine that provides stable
and secure extended cell storage for primary tissue isolates and constructs and prepared …

Cryopreservation of semen in domestic animals: A review of current challenges, applications, and prospective strategies

M Sharafi, SM Borghei-Rad, M Hezavehei, A Shahverdi… - Animals, 2022 - mdpi.com
Simple Summary Here we present a comprehensive review of the current literature
describing mechanisms of damage incurred by cryopreservation, and we highlight several …

Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse

BA Ball - Animal reproduction science, 2008 - Elsevier
Oxidative stress is an important component of the cytopathology of equine spermatozoa
undergoing storage as liquid or frozen semen. Damage to chromatin, membranes and …

The cryobiology of spermatozoa

JD Benson, EJ Woods, EM Walters, JK Critser - Theriogenology, 2012 - Elsevier
The impact of successful cryopreservation of spermatozoa can be found in many fields,
including agriculture, laboratory animal medicine, and human assisted reproduction …

Freezing injury: the special case of the sperm cell

GJ Morris, E Acton, BJ Murray, F Fonseca - Cryobiology, 2012 - Elsevier
The cellular damage that spermatozoa encounter at rapid rates of cooling has often been
attributed to the formation of intracellular ice although no convincing evidence of intracellular …

Sperm membrane behaviour during cooling and cryopreservation

H Sieme, H Oldenhof… - Reproduction in Domestic …, 2015 - Wiley Online Library
Contents Native sperm is only marginally stable after collection. Cryopreservation of semen
facilitates transport and storage for later use in artificial reproduction technologies, but …

The Grand Challenges of Organ Banking: Proceedings from the first global summit on complex tissue cryopreservation

JK Lewis, JC Bischof, I Braslavsky, KGM Brockbank… - Cryobiology, 2016 - Elsevier
Abstract The first Organ Banking Summit was convened from Feb. 27-March 1, 2015 in Palo
Alto, CA, with events at Stanford University, NASA Research Park, and Lawrence Berkeley …

[PDF][PDF] Lipid peroxidation, assessed with BODIPY-C11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic-like …

CO Ferrusola, LG Fernández, JM Morrell… - Reproduction, 2009 - academia.edu
Lipid peroxidation (LPO) of stallion spermatozoa was assessed in fresh semen and in
samples of the same ejaculates after freezing and thawing. Particular attention was paid to …

The Impact of Sperm Metabolism during In Vitro Storage: The Stallion as a Model

Z Gibb, RJ Aitken - BioMed research international, 2016 - Wiley Online Library
In vitro sperm storage is a necessary part of many artificial insemination or in vitro
fertilization regimes for many species, including the human and the horse. In many situations …

Dissecting the molecular damage to stallion spermatozoa: the way to improve current cryopreservation protocols?

FJ Pena, BM García, JC Samper, IM Aparicio, JA Tapia… - Theriogenology, 2011 - Elsevier
We review recent developments in the technology of freezing stallion sperm, paying special
attention to the molecular lesions that spermatozoa suffer during freezing and thawing, such …