Droplet-based digital PCR (ddPCR) and its applications

Y Hou, S Chen, Y Zheng, X Zheng, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

Droplet digital PCR applications in the tuberculosis world

R Nyaruaba, C Mwaliko, KK Kering, H Wei - Tuberculosis, 2019 - Elsevier
Droplet digital polymerase chain reaction (ddPCR) is a third generation of polymerase chain
reaction (PCR) that enables the exact quantification of nucleic acid targets within a sample …

Plant systems biology at the single-cell level

M Libault, L Pingault, P Zogli, J Schiefelbein - Trends in Plant Science, 2017 - cell.com
Our understanding of plant biology is increasingly being built upon studies using 'omics and
system biology approaches performed at the level of the entire plant, organ, or tissue …

Copy number alterations as novel biomarkers and therapeutic targets in colorectal cancer

ES Tan, TC Knepper, X Wang, JB Permuth, L Wang… - Cancers, 2022 - mdpi.com
Simple Summary Copy number alterations (CNAs) occur due to changes to DNA structure
that lead to the gain/amplification or loss/deletion of copies of DNA sections from a normal …

Digital PCR: What relevance to plant studies?

C Morcia, R Ghizzoni, C Delogu, L Andreani… - Biology, 2020 - mdpi.com
Simple Summary Digital PCR is a third-generation technology based on the subdivision of
the analytical sample into numerous partitions that are amplified individually. This review …

Experimental evaluation of genomic selection prediction for rust resistance in sugarcane

MS Islam, PH McCord, MO Olatoye, L Qin… - The Plant …, 2021 - Wiley Online Library
The total sugarcane (Saccharum L.) production has increased worldwide; however, the rate
of growth is lower compared with other major crops, mainly due to a plateauing of genetic …

Application of droplet digital PCR to determine copy number of endogenous genes and transgenes in sugarcane

Y Sun, PA Joyce - Plant cell reports, 2017 - Springer
Key message Droplet digital PCR combined with the low copy ACT allele as endogenous
reference gene, makes accurate and rapid estimation of gene copy number in Q208 A and …

Review on the evolution in DNA-based techniques for molecular characterization and authentication of GMOs

Z Liang, L Ding, M Tang, X Wang, X Chen, J Xu… - Microchemical …, 2024 - Elsevier
Genetically modified (GM) foods have not only improved the yield and quality of food but
also raised public concerns about the safety of GM foods. However, with the continuous …

Droplet Digital PCR (ddPCR) Analysis for Detecting Shiga-Toxin-Producing Escherichia coli (STEC)

A Mancusi, A Fulgione, S Girardi, O Di Maro… - Applied Sciences, 2022 - mdpi.com
Verocytotoxin-producing Escherichia coli, also referred to as Shiga-toxin-producing
Escherichia coli (STEC), can be transmitted to humans through person-to-person contact …

[HTML][HTML] 数字PCR 在功能核酸精准检测中的研究进展

刘晓, 朱鹏宇, 王垚, 朱水芳, 付伟 - 生物技术通报, 2018 - html.rhhz.net
数字PCR 是近年来迅速发展起来的一种定量分析技术. 该技术结果判定不依赖于扩增曲线的
循环阈值(Ct), 不受扩增效率的影响, 具有很好的准确度和重现性, 并且可以实现绝对定量分析 …