In just a few years, microarrays have gone from obscurity to being almost ubiquitous in
biological research. At the same time, the statistical methodology for microarray analysis has …

We review the fundamental principles of statistical experimental design, and their
application to quantitative mass spectrometry-based proteomics. We focus on class …

The ability to form memories is a prerequisite for an organism's behavioral adaptation to
environmental changes. At the molecular level, the acquisition and maintenance of memory …

Costs can hamper the evaluation of the effectiveness of new biomarkers. Analysis of smaller
numbers of pooled specimens has been shown to be a useful cost-cutting technique. The …

Background Massively parallel cDNA sequencing (RNA-seq) experiments are gradually
superseding microarrays in quantitative gene expression profiling. However, many …

Over 15% of the data sets catalogued in the Gene Expression Omnibus Database involve
RNA samples that have been pooled before hybridization. Pooling affects data quality and …

Circadian rhythms are approximate 24-h behavioral and physiological cycles that function to
prepare an organism for daily environmental changes. The basic clock mechanism is a …

Playing a central role in the maintenance of hemostasis as well as in thrombotic disorders,
platelets contain a relatively diverse messenger RNA (mRNA) transcriptome as well as …

We assess the reliability of isobaric-tags for relative and absolute quantitation (iTRAQ),
based on different types of replicate analyses taking into account technical, experimental …

Heritable variation in regulatory or coding regions is the raw material for evolutionary
processes. The advent of microarrays has recently promoted examination of the extent of …