Intracellular mRNA transport and localized translation
Fine-tuning cellular physiology in response to intracellular and environmental cues requires
precise temporal and spatial control of gene expression. High-resolution imaging …
precise temporal and spatial control of gene expression. High-resolution imaging …
Real-time single-molecule imaging of transcriptional regulatory networks in living cells
Gene regulatory networks drive the specific transcriptional programmes responsible for the
diversification of cell types during the development of multicellular organisms. Although our …
diversification of cell types during the development of multicellular organisms. Although our …
An improved MS2 system for accurate reporting of the mRNA life cycle
The MS2–MCP system enables researchers to image multiple steps of the mRNA life cycle
with high temporal and spatial resolution. However, for short-lived mRNAs, the tight binding …
with high temporal and spatial resolution. However, for short-lived mRNAs, the tight binding …
ATPase activity of the DEAD-box protein Dhh1 controls processing body formation
Translational repression and mRNA degradation are critical mechanisms of
posttranscriptional gene regulation that help cells respond to internal and external cues. In …
posttranscriptional gene regulation that help cells respond to internal and external cues. In …
Imaging mRNA in vivo, from birth to death
RNA is the fundamental information transfer system in the cell. The ability to follow single
messenger RNAs (mRNAs) from transcription to degradation with fluorescent probes gives …
messenger RNAs (mRNAs) from transcription to degradation with fluorescent probes gives …
Stem–loop RNA labeling can affect nuclear and cytoplasmic mRNA processing
S Heinrich, CL Sidler, CM Azzalin, K Weis - Rna, 2017 - rnajournal.cshlp.org
The binding of sequence-specific RNA-interacting proteins, such as the bacteriophage MS2
or PP7 coat proteins, to their corresponding target sequences has been extremely useful …
or PP7 coat proteins, to their corresponding target sequences has been extremely useful …
CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos
Background Understanding gene transcription and mRNA-protein (mRNP) dynamics in
single cells in a multicellular organism has been challenging. The catalytically dead …
single cells in a multicellular organism has been challenging. The catalytically dead …
Imaging of DNA and RNA in living eukaryotic cells to reveal spatiotemporal dynamics of gene expression
This review focuses on imaging DNA and single RNA molecules in living cells to define
eukaryotic functional organization and dynamic processes. The latest advances in …
eukaryotic functional organization and dynamic processes. The latest advances in …
Technologies enabling single-molecule super-resolution imaging of mRNA
The transient nature of RNA has rendered it one of the more difficult biological targets for
imaging. This difficulty stems both from the physical properties of RNA as well as the …
imaging. This difficulty stems both from the physical properties of RNA as well as the …
Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines
Large RNAs and ribonucleoprotein complexes have powerful therapeutic potential, but
effective cell-targeted delivery tools are limited. Aptamers that internalize into target cells can …
effective cell-targeted delivery tools are limited. Aptamers that internalize into target cells can …