DNA replicases from a bacterial perspective
CS McHenry - Annual review of biochemistry, 2011 - annualreviews.org
Bacterial replicases are complex, tripartite replicative machines. They contain a polymerase,
polymerase III (Pol III), a β2 processivity factor, and a DnaX complex ATPase that loads β2 …
polymerase III (Pol III), a β2 processivity factor, and a DnaX complex ATPase that loads β2 …
The E. coli DNA replication fork
DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds
bidirectionally, resulting in two replication forks that travel in opposite directions from the …
bidirectionally, resulting in two replication forks that travel in opposite directions from the …
Single-molecule visualization of fast polymerase turnover in the bacterial replisome
JS Lewis, LM Spenkelink, S Jergic, EA Wood… - Elife, 2017 - elifesciences.org
The Escherichia coli DNA replication machinery has been used as a road map to uncover
design rules that enable DNA duplication with high efficiency and fidelity. Although the …
design rules that enable DNA duplication with high efficiency and fidelity. Although the …
[HTML][HTML] DNA replication is the target for the antibacterial effects of nonsteroidal anti-inflammatory drugs
Evidence suggests that some nonsteroidal anti-inflammatory drugs (NSAIDs) possess
antibacterial properties with an unknown mechanism. We describe the in vitro antibacterial …
antibacterial properties with an unknown mechanism. We describe the in vitro antibacterial …
Single-molecule studies of fork dynamics in Escherichia coli DNA replication
NA Tanner, SM Hamdan, S Jergic, KV Loscha… - Nature structural & …, 2008 - nature.com
We present single-molecule studies of the Escherichia coli replication machinery. We
visualize individual E. coli DNA polymerase III (Pol III) holoenzymes engaging in primer …
visualize individual E. coli DNA polymerase III (Pol III) holoenzymes engaging in primer …
Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes
Current challenges in the field of structural genomics point to the need for new tools and
technologies for obtaining structures of macromolecular protein complexes. Here, we …
technologies for obtaining structures of macromolecular protein complexes. Here, we …
cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ
R Fernandez-Leiro, J Conrad, SHW Scheres… - Elife, 2015 - elifesciences.org
The replicative DNA polymerase PolIIIα from Escherichia coli is a uniquely fast and
processive enzyme. For its activity it relies on the DNA sliding clamp β, the proofreading …
processive enzyme. For its activity it relies on the DNA sliding clamp β, the proofreading …
A direct proofreader–clamp interaction stabilizes the Pol III replicase in the polymerization mode
S Jergic, NP Horan, MM Elshenawy, CE Mason… - The EMBO …, 2013 - embopress.org
Processive DNA synthesis by the αεθ core of the Escherichia coli Pol III replicase requires it
to be bound to the β2 clamp via a site in the α polymerase subunit. How the ε proofreading …
to be bound to the β2 clamp via a site in the α polymerase subunit. How the ε proofreading …
Polymerase exchange on single DNA molecules reveals processivity clamp control of translesion synthesis
JE Kath, S Jergic, JMH Heltzel… - Proceedings of the …, 2014 - National Acad Sciences
Translesion synthesis (TLS) by Y-family DNA polymerases alleviates replication stalling at
DNA damage. Ring-shaped processivity clamps play a critical but ill-defined role in …
DNA damage. Ring-shaped processivity clamps play a critical but ill-defined role in …
Peptide-based covalent inhibitors bearing mild electrophiles to target a conserved his residue of the bacterial sliding clamp
Peptide-based covalent inhibitors targeted to nucleophilic protein residues have recently
emerged as new modalities to target protein–protein interactions (PPIs) as they may provide …
emerged as new modalities to target protein–protein interactions (PPIs) as they may provide …