Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

This review summarizes the current state of methods and results achievable by cryo-electron
microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to …

Cryofixation has proven to be the gold standard for efficient preservation of native cell
ultrastructure compared to chemical fixation, but this approach is not widely used in …

Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …

Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent cause of familial
Parkinson's disease. LRRK2 is a multi-domain protein containing a kinase and GTPase …

Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the
structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo …

Although viruses are extremely diverse in shape and size, evolution has led to a limited
number of viral classes or lineages, which is probably linked to the assembly constraints of a …

Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular
structures in their true structural context. However, current commercial cryo-fluorescence …