In the past two decades, significant advances have been made in single-molecule detection
which enables the direct observation of single biomolecules at work in real time and under …

A wide-field fluorescence microscope with a double-helix point spread function (PSF) is
constructed to obtain the specimen's three-dimensional distribution with a single snapshot …

Single-molecule localization microscopy, typically based on total internal reflection
illumination, has taken our understanding of protein organization and dynamics in cells …

Nanoscale localization of point emitters is critical to several methods in optical fluorescence
microscopy, including single-molecule super-resolution imaging and tracking. While the …

Optical aberrations degrade image quality in fluorescence microscopy, including for single-
molecule based techniques. These depend on post-processing to localize individual …

Single-molecule localization microscopy has developed into a widely used technique to
overcome the diffraction limit and enables 3D localization of single-emitters with nanometer …

An approach for designing purely refractive optical elements that generate engineered, multi-
order-helix point spread functions (PSFs) with large peak separation for passive, optical …

The vortex self-imaging (SI) implemented in optical imaging systems and its usage for a
robust axial localization of point-like objects are presented. The vortex SI is used to generate …

Point spread function (PSF) engineering has met with lots of interest in various optical
imaging techniques, including super-resolution microscopy, microparticle tracking, and …

Rotating point spread function (PSF) provides a novel approach to detect three-dimensional
position. As the optical aberration of a system can hardly be removed completely, effects of …