[HTML][HTML] CRISPR-Cas9 genome editing approaches in filamentous fungi and oomycetes

M Schuster, R Kahmann - Fungal Genetics and Biology, 2019 - Elsevier
Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

Transcriptional activation of biosynthetic gene clusters in filamentous fungi

L Mózsik, R Iacovelli, RAL Bovenberg… - … in Bioengineering and …, 2022 - frontiersin.org
Filamentous fungi are highly productive cell factories, many of which are industrial
producers of enzymes, organic acids, and secondary metabolites. The increasing number of …

Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis

J Kämper, R Kahmann, M Bölker, LJ Ma, T Brefort… - Nature, 2006 - nature.com
Ustilago maydis is a ubiquitous pathogen of maize and a well-established model organism
for the study of plant–microbe interactions. This basidiomycete fungus does not use …

Genome editing in Ustilago maydis using the CRISPR–Cas system

M Schuster, G Schweizer, S Reissmann… - Fungal Genetics and …, 2016 - Elsevier
This communication describes the establishment of the type II bacterial CRISPR–Cas9
system to efficiently disrupt target genes in the fungal maize pathogen Ustilago maydis. A …

Modular synthetic biology toolkit for filamentous fungi

L Mózsik, C Pohl, V Meyer, RAL Bovenberg… - ACS Synthetic …, 2021 - ACS Publications
Filamentous fungi are highly productive cell factories, often used in industry for the
production of enzymes and small bioactive compounds. Recent years have seen an …

A dispensable paralog of succinate dehydrogenase subunit C mediates standing resistance towards a subclass of SDHI fungicides in Zymoseptoria tritici

D Steinhauer, M Salat, R Frey, A Mosbach… - PLoS …, 2019 - journals.plos.org
Succinate dehydrogenase inhibitor (SDHI) fungicides are widely used for the control of a
broad range of fungal diseases. This has been the most rapidly expanding fungicide group …

Ustilago maydis, a new fungal model system for cell biology

G Steinberg, J Perez-Martin - Trends in cell biology, 2008 - cell.com
The use of fungal model systems, such as Saccharomyces cerevisisae and
Schizosaccharomyces pombe, has contributed enormously to our understanding of …

Establishing a versatile Golden Gate cloning system for genetic engineering in fungi

M Terfrüchte, B Joehnk, R Fajardo-Somera… - Fungal Genetics and …, 2014 - Elsevier
The corn pathogen Ustilago maydis is a well-studied fungal model organism. Along with a
broad set of experimental tools, versatile strategies for the generation of gene replacement …

Tet-on, or Tet-off, that is the question: advanced conditional gene expression in Aspergillus

F Wanka, T Cairns, S Boecker, C Berens… - Fungal Genetics and …, 2016 - Elsevier
In Aspergillus, controlled gene expression is often achieved using the reverse tetracycline-
controlled transactivator (rtTA) dependent Tet-on system, whereby transcription is activated …

The biotechnological use and potential of plant pathogenic smut fungi

M Feldbrügge, R Kellner, K Schipper - Applied microbiology and …, 2013 - Springer
Plant pathogens of the family Ustilaginaceae parasitise mainly on grasses and cause smut
disease. Among the best characterised members of this family are the covered smut fungus …