Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

There remains an urgent need for novel therapeutic strategies to treat metastatic cancer,
which results in over 8 million deaths annually worldwide. Following secretion, exosomes …

Since techniques for cardiomyocyte isolation were first developed 35years ago, experiments
on single myocytes have yielded great insight into their cellular and sub-cellular physiology …

This protocol describes a method of genetic transformation for the rodent malaria parasite
Plasmodium berghei with a high transfection efficiency of 10− 3–10− 4. It provides methods …

Adult mammalian cardiomyocytes are considered terminally differentiated and incapable of
proliferation. Consequently, acutely injured mammalian hearts do not regenerate, they scar …

Cancer is one of the primary causes of death worldwide, and its morbidity and mortality rates
are increasing rapidly. However, standard treatment modalities (surgery, radiotherapy …

Brain-derived neurotrophic factor (BDNF), after activity-dependent secretion from neurons,
modulates critical nervous system functions. Recently, a variant in the human bdnf gene …

The use of transfection in the study of the biology of malaria parasites has been limited due
to poor transfection efficiencies (frequency of 10− 6 to 10− 9) and a paucity of selection …

In the past 4 years, RNA interference (RNAi) has become widely used as an experimental
tool to analyse the function of mammalian genes, both in vitro and in vivo. By harnessing an …

Intracellular delivery is considered an indispensable process for various studies, ranging
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …