Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Guidelines for the use of flow cytometry and cell sorting in immunological studies*

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2017 - Wiley Online Library
From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

Nuclear cytometry and chromatin organization

PJ Smith, Z Darzynkiewicz, RJ Errington - Cytometry Part A, 2018 - Wiley Online Library
The nuclear‐targeting chemical probe, for the detection and quantification of DNA within
cells, has been a mainstay of cytometry—from the colorimetric Feulgen stain to smart …

Artificial shear stress effects on leukocytes at a biomaterial interface

G Radley, I Laura Pieper, BR Thomas… - Artificial …, 2019 - Wiley Online Library
Medical devices, such as ventricular assist devices (VADs), introduce both foreign materials
and artificial shear stress to the circulatory system. The effects these have on leukocytes and …

Flow cytometric identification and enumeration of monocyte subsets in bovine and water Buffalo peripheral blood

F Grandoni, D Fraboni, B Canonico, S Papa… - Current …, 2023 - Wiley Online Library
Monocytes are innate immune system key players with pivotal roles during infection and
inflammation. They migrate into tissues and differentiate into myeloid effect cells …

The effect of ventricular assist device‐associated biomaterials on human blood leukocytes

G Radley, IL Pieper, CA Thornton - Journal of Biomedical …, 2018 - Wiley Online Library
Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients
with advanced stage heart failure. These devices remain susceptible to adverse events …

Single-cell digital lysates generated by phase-switch microfluidic device reveal transcriptome perturbation of cell cycle

Y Chen, J Millstein, Y Liu, GY Chen, X Chen, A Stucky… - ACS …, 2018 - ACS Publications
With conventional gene expression profiling, information concerning cellular heterogeneity
is often lost in the physical mixing and averaging of millions of cells. Single-cell …

Improving single-cell transcriptome sequencing efficiency with a microfluidic phase-switch device

B Zhang, H Xu, Y Huang, W Shu, H Feng, J Cai… - Analyst, 2019 - pubs.rsc.org
In this paper, we present a novel method to improve the efficiency of single-cell
transcriptome sequencing for analyzing valuable cell samples. The microfluidic device we …

Ovine leukocyte microparticles generated by the centrimag ventricular assist device in vitro

IL Pieper, G Radley, A Christen, S Ali, O Bodger… - Artificial …, 2018 - Wiley Online Library
Ventricular assist devices (VADs) are a life‐saving form of mechanical circulatory support in
heart failure patients. However, VADs have not yet reached their full potential due to the …

DAPI 染色法在流式细胞术中去除死细胞影响的探讨.

许雅苹, 郑晓辉, 房纯正, 翁乐斌… - Progress in Modern …, 2019 - search.ebscohost.com
To explore the method of DAPI staining in the process of flow cytometry to remove dead cells
and eliminate the influence of dead cells on flow cytometry results. An orthotopic …