RNA silencing movement in plants

K Kalantidis, HT Schumacher, T Alexiadis… - Biology of the …, 2008 - Wiley Online Library
Higher eukaryotes have developed a mechanism of sequence‐specific RNA degradation
which is known as RNA silencing. In plants and some animals, similar to the nematode …

Agroinfiltration mediated scalable transient gene expression in genome edited crop plants

M Kaur, P Manchanda, A Kalia, FK Ahmed… - International Journal of …, 2021 - mdpi.com
Agrobacterium-mediated transformation is one of the most commonly used genetic
transformation method that involves transfer of foreign genes into target plants …

An efficient system to detect protein ubiquitination by agroinfiltration in Nicotiana benthamiana

L Liu, Y Zhang, S Tang, Q Zhao, Z Zhang… - The plant …, 2010 - Wiley Online Library
The ubiquitination proteasome pathway has been demonstrated to regulate all plant
developmental and signaling processes. E3 ligase/substrate‐specific interactions and …

Induction of Silencing in Plants by High-Pressure Spraying of In vitro-Synthesized Small RNAs

A Dalakouras, M Wassenegger, JN McMillan… - Frontiers in plant …, 2016 - frontiersin.org
In this report, we describe a method for the delivery of small interfering RNAs (siRNAs) into
plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a …

RNAi‐induced silencing of gene expression in strawberry fruit (Fragaria × ananassa) by agroinfiltration: a rapid assay for gene function analysis

T Hoffmann, G Kalinowski, W Schwab - The Plant Journal, 2006 - Wiley Online Library
Intron‐containing constructs encoding self‐complementary 'hairpin'RNA (ihpRNA) have the
potential to efficiently silence genes in a range of plant species. In this study we demonstrate …

The key role of terminators on the expression and post‐transcriptional gene silencing of transgenes

FF de Felippes, M McHale, RL Doran… - The Plant …, 2020 - Wiley Online Library
Transgenes have become essential to modern biology, being an important tool in functional
genomic studies and also in the development of biotechnological products. One of the major …

Agrobacterium-Mediated Transient Gene Expression and Silencing: A Rapid Tool for Functional Gene Assay in Potato

PB Bhaskar, M Venkateshwaran, L Wu, JM Ané… - PLoS …, 2009 - journals.plos.org
Potato is the third most important food crop worldwide. However, genetic and genomic
research of potato has lagged behind other major crops due to the autopolyploidy and …

Induction of transgene suppression in plants via external application of synthetic dsRNA

AS Dubrovina, OA Aleynova, AV Kalachev… - International Journal of …, 2019 - mdpi.com
Recent investigations show that exogenously applied small interfering RNAs (siRNA) and
long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants …

RNA interference: A promising biotechnological approach to combat plant pathogens, mechanism and future prospects

A Ali, M Shahbaz, F Ölmez, N Fatima… - World Journal of …, 2024 - Springer
Plant pathogens and other biological pests represent significant obstacles to crop Protection
worldwide. Even though there are many effective conventional methods for controlling plant …

Intron splicing suppresses RNA silencing in Arabidopsis

M Christie, LJ Croft, BJ Carroll - The Plant Journal, 2011 - Wiley Online Library
Silencing of introduced transgenes constitutes a major bottleneck in the production of
transgenic crops. Commonly, these transgenes contain no introns, a feature shared with …