Genetic improvement of cattle around the globe has been, and will continue to be, an
important driver of animal agriculture sustainability. There are several reproductive and …

Selecting beneficial DNA variants is the main goal of animal breeding. However, this
process is inherently inefficient because each animal only carries a fraction of all desirable …

The significance of donor cell differentiation status for successful cloning by somatic cell
nuclear transfer (SCNT) is unclear. Here, we cloned a new species, red deer (Cervus …

Authentic induced pluripotent stem cells (iPSCs), capable of giving rise to all cell types of an
adult animal, are currently only available in mouse. Here, we report the first generation of …

The inner cell mass (ICM) of mammalian blastocysts consists of pluripotent epiblast and
hypoblast lineages, which develop into embryonic and extraembryonic tissues, respectively …

Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle
synchronization of donor cells, enucleation, cell fusion, oocyte activation and embryo …

Cells in the mammalian blastocyst segregate into three distinct lineages, namely,
trophoblast, hypoblast, and epiblast. During development, these will form extraembryonic …

Contents Despite more than a decade of research efforts, farm animal cloning by somatic
cell nuclear transfer (SCNT) is still frustratingly inefficient. Inefficiency manifests itself at …

Background Cloning of cattle by somatic cell nuclear transfer (SCNT) is associated with a
high incidence of pregnancy failure characterized by abnormal placental and foetal …

In this study, we analyzed the effects of the cloned embryo aggregation on in vitro embryo
development and embryo quality by measuring blastocyst diameter and cell number, DNA …